摘要
Abstract
Objective To investigate the effect of doxycycline on the renal interstitial fibrosis in rats and its possible mechanism. Methods The unilateral ureteral obstruction ( UUO ) was adopted to create the renal tubular interstitial fibrosis model in rats. 72 normal female Sprague-Dawley rats were randomly divided into the sham-operation group, UUO model group and doxycycline treatment [ 8 mg/ ( kg·d ) ] group. 6 rats in each group were respectively sacrificed on 7, 14, 21 d after surgery. Immunohistochemistry was adopted to detect the expression of transforming growth factor-β1 ( TGF-β1 ) and matrix metalloproteinase-2 ( MMP-2 ) in the obstructed renal tissue. The HE staining and the Masson staining were performed to evaluate the renal pathological status in each group. Results The expression of MMP-2 at different time points in the UUO model group was significantly lower than that in the sham-operation group ( P<0. 05 ) , and the expression of MMP-2 at different time points in the doxycycline group was significantly lower than that in the UUO model group ( P<0. 05 ) , the expression of MMP-2 on postoperative 14, 21 d in the doxycycline group were lower than that on postoperative 7 d. The expression of TGF-β1 at different time points in the UUO model group was significantly higher than that in the sham-operated group ( P<0. 05 ) , but the expression of TGF-β1 on postoperative 7 d in the doxycycline group was significantly lower than that in the UUO model group, which on postoperative 14, 21 d in the doxycycline group was significantly higher than that an post-operative 7 d. Conclusion Doxycycline has a protective effect on the early tubulointerstitial fibrosis.关键词
转化生长因子-β1/基质金属蛋白酶2/肾间质纤维化/多西环素Key words
TGF-β1/MMP-2/renal interstitial fibrosis/doxycycline分类
医药卫生
