中国药业Issue(19)：37-37,38,2.
蒙古山萝卜酸对实验性肝纤维化大鼠血清基质金属蛋白酶－1及其抑制因子表达水平的影响
Effect of Mongolia Scabious Acid on Serum MMP -1 and TIMP -1 Expression Levels in Experimental Liver Fibrosis Rats
摘要
Abstract
Objective To investigate the effect of Mongolia scabious acid ( MSA ) on the expression levels of serum matrix metalloproteinas-es-1 ( MMP-1 ) and tissue inhibitor of metalloproteinases-1 ( TIMP-1 ) in the experimental liver fibrosis rats. Methods 50 rats ( male and female half ) were divided into the control group, model group, positive control group ( colchicine 1. 5 mg/kg ) and high- and low-dose groups of tested drug ( MSA 240, 30 mg/kg ) , 10 rats in each group, except the control group, 25% CCl4 peanut oil1 mL/25 kg was given by subcutaneous injection, once per 4 d with a total of 30 times. After 4 times of injection, the positive control group, high- and low-dose groups of test drug began to give the test drug by intragastric administration, once daily. After the injection of 30 times, the anesthetic laparotomy was performed and blood was collected from rat abdominal aorta for separating serum. Then the ELISA determination was performed. Results Serum MMP-1 and TIMP-1 in the model group had a small amount of expression, the MMP-1 expression level in the model group was obviously decreased and the TIMP-1 expression was significantly increased compared with the control group ( P<0. 05 );the MMP-1 expression level in the positive medication control group had no obvious change and the TIMP-1 level was signifi-cantly decreased;the MMP-1 expression level in the high-dose group of test drug was significantly increased obviously and the expres-sion level of TIMP-1 was significantly reduced compared with the model group ( P<0. 05 );the low-dose group of test drug had no ef-fects on the expression of MMP-1 and TIMP-1. Conclusion MSA participates in the anti-liver fibrosis regulating effect in imbalance of MMP-1/TIMP-1 expression level in rat, which is one of the mechanisms of prevention and treatment of liver fibrosis.关键词
肝纤维化/免疫组化/基质金属蛋白酶-1/基质金属蛋白酶抑制因子-1Key words
liver fibrosis/ELISA/MMP-1/TIMP-1分类
医药卫生引用本文复制引用
杨宏昕,董珉翔,米裕青,白音夫..蒙古山萝卜酸对实验性肝纤维化大鼠血清基质金属蛋白酶－1及其抑制因子表达水平的影响[J].中国药业,2014,(19):37-37,38,2.基金项目
国家自然科学基金，项目编号81260659。 （）
