中国药业Issue(19)：42-43,44,3.
高效液相色谱法测定鲑鱼降钙素混悬型气雾剂的含量
Determination of Salmon Calcitonin in Salmon Calcitonin Suspension Aerosol by HPLC
吴涵 1陈龙楷 1单紫筠 1潘昕 1吴传斌1
作者信息
- 1. 中山大学药学院·广东省高校创新药物制剂工程技术研究中心，广东 广州 510006
- 折叠
摘要
Abstract
Objectiv To establish a method for the content determination of salmon calcitonin in Salmon Calcitonin Suspension Aerosol. Methods The chromatographic column was the Phenomenex Jupiter 300 Å C18 column ( 250 mm × 4. 6 mm, 5μm ) . The mobile phase A was 0. 02 mol/L tetramethyl ammonium hydroxide solution ( pH=2. 5 ) and the mobile phase B was acetonitrile. The samples were determined with binary gradient elution at a flow rate of 1. 0 mL/min and the UV detection wavelength at 220 nm. The column temperature was at 40℃. Results There was a good linear relationship between the peak area and the salmon calcitonin concentra-tion within the range of 0. 8-50 μg/mL, and the regression equation was A=4. 508 7 × 104ρ-7. 394 8 × 103, r=0. 999 7 ( n=7). The average recovery rate was 98. 24%, RSD=1. 18%. The average content of salmon calcitonin in salmon calcitonin suspension Aerosol was 1. 41 mg. Conclusion The HPLC method is accurate, reliable, easy operation and suitable for the content determination of Salmon Calci-tonin Suspension Aerosol.关键词
鲑鱼降钙素/压力定量气雾剂/高效液相色谱法/含量测定Key words
salmon calcitonin/pressurized metered dose inhaler ( pMDI )/HPLC/content determination分类
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吴涵,陈龙楷,单紫筠,潘昕,吴传斌..高效液相色谱法测定鲑鱼降钙素混悬型气雾剂的含量[J].中国药业,2014,(19):42-43,44,3.
