中国药业Issue(20)：38-40,3.
薄层色谱生物自显影法筛选新疆莫合烟天然乙酰胆碱酯酶抑制剂的活性成分
TLC Bioautographic Method for Activity Screening of Natural Acetyl Cholinesterase In-hibitors from Xinjiang Mohe-Tobacco
摘要
Abstract
Objective To explain nicotine in tobacco can reduce the incidence of degenerative diseases,perform the activity screening of acetyl cholinesterase inhibitors of Xinjiang's unique Mohe-tobacco for illuminating the action mechanism. Methods The thin layer chromatography(TLC)bioautographic method combining TLC with biological activity assay was used to conduct the activity screening of extract solution of Xinjiang Mohe-tobacco. Results Nicotine in the extract solution of Xinjiang Mohe-tobacco appeared the white spots on the purple background by the TLC bioautography,indicating that which possesses the activity,but the white spots were weak, indicating that the nicotine content in the extract solution was unable to reach the significant inhibiting concentration,its activity was lower than that of the reference substance huperzine A;the DPPH development method revealed that the extraction solution of Xinjiang Mohe-tobacco simultaneously possesses the better antioxidant activity. Conclusion Nicotine in Xinjiang Mohe-tobacco can be com-bined with acetyl cholinesterase and inhibits its activity. The TLC bioautographic method is easy to operate with high sensitivity and specificity in the activity screening for natural acetyl cholinesterase inhibitor.关键词
新疆莫合烟/生物自显影/活性筛选/乙酰胆碱酯酶抑制剂/抗氧化Key words
Xinjiang Mohe-tobacco/bioautographic/activity screening/acetyl cholinesterase inhibitors/antioxidation分类
医药卫生引用本文复制引用
王健,朱萍萍,倪国柱,温琳豫,支玲,胡曙晨..薄层色谱生物自显影法筛选新疆莫合烟天然乙酰胆碱酯酶抑制剂的活性成分[J].中国药业,2014,(20):38-40,3.基金项目
新疆维吾尔自治区科技重大专项，项目编号201130105-2。 （）
