中国药业Issue(20)：61-62,2.
香草醛硫酸法测定榆黄喷雾剂中缩合鞣质含量
Determination of Condensed Tannins Content in Yuhuang Spray by Vanillin-H2SO4 Method
王建平 1颜耀东 1杨云 1张兰欣 1惠金玲 1马长勇 1张学慧 1迟平 1刘茵 1张娟1
作者信息
- 1. 中国人民解放军空军航空医学研究所附属医院，北京 100089
- 折叠
摘要
Abstract
Objective To establish the quantitative determination method of condensed tannins in Yuhuang Spray. Methods The test so-lution was added with 3% vanillin methanol solution 5 mL and 30% concentrated sulfuric acid methanol solution 5 mL in turn,then which was developed by keeping away from light for 20 min,the absorbance was detected at 500 nm by spectrophotometry and the condensed tannin content in the sample was calculated. Results The concentration of catechins reference substance had a good linear relationship with the absorbance in the range of 0. 03-0. 3 g/L,and the regression equation was Y=3. 065 2 X+0. 025 7,r=0. 999 3 ( n=10 ) ,the average recovery rate was 99. 62%,RSD=1. 76%( n=6). Conclusion The vanillin-H2SO4 method is simple and rapid with good precision,stability,reproducibility and specificity and suitable for the determination of the condensed tannins content in Yuhuang Spray.关键词
儿茶素/鞣质/缩合鞣质/香草醛硫酸法/紫外分光光度法/榆黄喷雾剂/榆树皮Key words
catechin/condensed tannins/vanillin-H2SO4 method/UV spectrophotometry/Yuhuang Spray/elm bark分类
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王建平,颜耀东,杨云,张兰欣,惠金玲,马长勇,张学慧,迟平,刘茵,张娟..香草醛硫酸法测定榆黄喷雾剂中缩合鞣质含量[J].中国药业,2014,(20):61-62,2.
