反相高效液相色谱法测定保婴镇惊丸中大黄素和大黄酚含量OA
Content Determination of Emodin and Chrysophanol in Baoyingzhenjing Pill by RP-HPLC
目的：建立测定保婴镇惊丸中大黄素和大黄酚含量的反相高效液相色谱法。方法色谱柱为Zorbax SB－C18柱(250 mm ×4.6 mm，5μm)，流动相为甲醇－0.1%磷酸（85：15），检测波长为254 nm，柱温为35℃。结果大黄素、大黄酚进样量分别在0.0412~0.6180μg （ r=0.999999）和0.0906~1.3590μg（ r=0.999998）范围内与峰面积线性关系良好；平均回收率分别为97.02%和98.12%，RS…查看全部>>
Objective To establish a RP-HPLC method for the content determination of emodin and chrysophanol in Baoyingzhenjing Pill. Methods The analysis was performed on the Zorbax SB-C18 column ( 250 mm × 4. 6 mm,5 μm ) with methanol-0. 1% phosphoric acid(85 : 15) as the mobile phase. Emodin and chrysophanol were detected at 254 nm. The column temperature was 35 ℃. Results The linear range was 0. 041 2-0. 618 0 μg( r=0. 999 999)for emodin sample size and 0. 090 6-1. …查看全部>>
巩长芹
山东省临沂市食品药品检验检测中心，山东 临沂 276001
医药卫生
反相高效液相色谱法大黄素大黄酚保婴镇惊丸含量测定
RP-HPLCemodinchrysophanolBaoyingzhenjing Pillcontent determination
《中国药业》 2014 (22)
58-59,2
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