摘要
Abstract
Objective To establish a RP-HPLC method for the content determination of emodin and chrysophanol in Baoyingzhenjing Pill. Methods The analysis was performed on the Zorbax SB-C18 column ( 250 mm × 4. 6 mm,5 μm ) with methanol-0. 1% phosphoric acid(85 : 15) as the mobile phase. Emodin and chrysophanol were detected at 254 nm. The column temperature was 35 ℃. Results The linear range was 0. 041 2-0. 618 0 μg( r=0. 999 999)for emodin sample size and 0. 090 6-1. 359 0 μg( r=0. 999 998)for chrysophanol sample size,respectively. The average recovery rates of emodin and chrysophanol were 97. 02% and 98. 12%,RSD were 1. 16% and 0. 94% ( n=6 ) ,respectively. Conclusion This method is simple,rapid,accurate and suitable for determining the contents of emodin and chrysophanol in Baoyingzhenjin Pill.关键词
反相高效液相色谱法/大黄素/大黄酚/保婴镇惊丸/含量测定Key words
RP-HPLC/emodin/chrysophanol/Baoyingzhenjing Pill/content determination分类
医药卫生
