摘要
Abstract
Objective To establish a HPLC method for the determination of Cassia corymbosa glycoside content in Jingxuening Capules. Methods The analytical column was a Capcellpak-C18 ( 250 mm × 4. 6 mm, 5 μm ) . The mobile phase consisted of methanol-phosphate mixed solution ( dissolving disodium hydrogen phosphate 1. 8 g, potassium dihydrogen phosphate 2. 8 g and heptane sulfonic acid sodium 1. 0 g in water and adding water to 1L, adjusting to pH 6. 0 by phosphoric acid ) ( 25:75 ) . The detection wavelength was 335 nm. The column temperature was 30℃ and the flow rate was 1. 0 mL/min. Results The linear range of Cassia corymbosa glycoside was in the range of 0. 001 991-0. 995 5 μg, r=0. 999 6, the average recovery rate was 99. 08% with RSD 1. 88% ( n=9 ) . Conclusion This method has strong specificity and good repeatability, and is suitable for the content determination of Cassia corymbosa glycoside in Jingxuening Capules.关键词
经血宁胶囊/伞房决明苷/高效液相色谱法Key words
Jingxuening Capules/Cassia corymbosa glycoside/HPLC分类
医药卫生
