中国药业Issue(4)：34-36,3.
高效液相色谱法和紫外分光光度法测定呋喃西林含量的比较研究
Comparison Research of HPLC and UV Methods for Determining Nitrofurazone Content
摘要
Abstract
Objective To establish a high performance liquid chromatography ( HPLC ) method for determining the nitrofurazone content and to compare it with the ultraviolet ( UV ) method. Methods With octadecylsilane chemically bonded silica as the filler,0. 125% tri-ethylamine buffer solution-acetonitrile solution (85 :15) was used as the mobile phase. The flow rate was 1 mL/min and the wave-length of detection was 375nm by the UV method. Results The nitrofurazone contents of three nitrofurazone solution samples deter-mined by the HPLC method were 90. 21%,90. 73% and 89. 75% of the labeled amount,while those determined by the UV method were 95. 72%,94. 09% and 94. 73% of the labeled amount respectively. Conclusion The determined content of nitrofurazone solution samples by the UV method is slightly higher than that determined by the HPLC method,but the HPLC method is more suitable as the content determination method for the nitrofurazone solution.关键词
高效液相色谱法/紫外分光光度法/呋喃西林/含量测定Key words
HPLC/UV/nitrofurazone/content determination分类
医药卫生引用本文复制引用
吴诚,曹淼,张丽平,马萍..高效液相色谱法和紫外分光光度法测定呋喃西林含量的比较研究[J].中国药业,2015,(4):34-36,3.基金项目
2013年军队医疗机构制剂标准提高科研专项课题计划重点项目，项目编号13ZJZ22。 （）
