中国药业Issue(6)：38-39,40,3.
高效液相色谱法测定卡托普利缓释片中卡托普利含量
Determination of Captopril Content in Captopril Sustained-Release Tatlets by HPLC
隋译 1王云龙1
作者信息
- 1. 黑龙江省鸡西市食品药品检验检测中心，黑龙江 鸡西 158100
- 折叠
摘要
Abstract
Objective To establish a high performance liquid chromatography ( HPLC ) method for the direct determination of the captopril content in Captopril Sustained-Release Tatlets. Methods The chromatographic column was the Agilent Eclipse XDB-C18 column (250 mm × 4. 6 mm,5 μm)with 0. 01 mol/L ammonium dihydrogen phosphate aqueous solution(adjusting pH to 3. 0 ± 0. 05 with phos-phoric acid)-methanol-acetonitrile(65 :25 :15) as the mobile phase,the flow rate was 1. 0 mL/min and the detection wavelength was 215 nm. Results The mass concentration of captopril in the range of 0. 2-2. 0 μg/mL showed the good linear relation with the peak area( r=0. 999 9),the averaga recovery rate was 99. 34%,RSD=0. 52%( n=9). Conclusion The separation obtained by adopt-ing the established method is good and the detection results are accurate and reliable with good reproducibility.关键词
高效液相色谱法/卡托普利缓释片/卡托普利Key words
HPLC/Captopril Sustained-Release Tatlets/captopril分类
医药卫生引用本文复制引用
隋译,王云龙..高效液相色谱法测定卡托普利缓释片中卡托普利含量[J].中国药业,2015,(6):38-39,40,3.
