中国药业Issue(8)：45-47,48,4.
蒙药材山野豌豆总DNA提取方法研究﹡
Total DNA Extraction Methods of Mengolian Medicinal Material Vicia Amoena
摘要
Abstract
Objective To develop a quick and convenient method for the extraction of total DNA of Vicia amoena. Methods The total DNA from Vicia amoena was detected by adopting 4 kinds of method [ cetyltrimethylammonium bromide(CTAB),sodium dodecyl sulfonate (SDS),modified CTAB and modified SDS ] and its purity was detected by the agarose gel electrophoresis and the ultraviolet spec-trophotometer method. The best extraction method was screened out. Results Both the modified CTAB method and the modified SDS method extracted a high purity of total DNA from Vicia amoena,and the value of A260 and A280 was 1. 912 8 ± 0. 082 7 and 1. 876 7 ± 0. 040 8 respectively. The total DNA extracted by the modified CTAB method showed the bright band without tail phenomenon in elec-trophoresis. Conclusion The modified CTAB method is an effective method for extracting high quality total DNA from Vicia amoena.关键词
山野豌豆/DNA提取/蒙药材/CTAB法/SDS法Key words
Vicia amoena/DNA extraction/Mongolian medicinal material/CTAB method/SDS method分类
医药卫生引用本文复制引用
高优恒,高俊杰,王海鹏,李骁..蒙药材山野豌豆总DNA提取方法研究﹡[J].中国药业,2015,(8):45-47,48,4.基金项目
内蒙古科技厅科技计划项目，项目编号20080502；内蒙古医科大学科技百万工程项目，项目编号kjbw2012009。 （）
