中国药业Issue(9)：18-19,20,3.
反相高效液相色谱法同时测定大鼠血清中多索茶碱和甲泼尼龙的质量浓度
Simultaneous Determination of Doxofylline and Methylprednisolone Concentrations in Rat Serum by RP-HPLC
摘要
Abstract
Objective To establish a RP-HPLC method for the simultaneous determination of doxofylline and methylprednisolone con-tents in rat serum. Methods Hydrocortisone was selected as the internal standard. The chromatographic separation of analytes was achieved on the Diamonsil C18 column(150mm × 4. 6 mm,5 μm) at the temperature of 30 ℃, using the mobile phase consisting of methanol-0. 5% acetic acid solution ( 54 :46 ) at a flow rate of 1. 0 mL/min . The detection wavelength was set at 260 nm. Results Doxofylline, methylprednisolone and endogenous substances in serum were separated well. The linear ranges of doxofylline and methylpred-nisolone were 0. 5-40 μg/mL ( r=0. 999 5 ) and 0. 3-24 μg/mL ( r=0. 999 6 ) . The inter-day and intra-day RSD of doxofylline and methylprednisolone were all less than 10% ( n=5 ) . The average recovery rates were 93. 49% ( RSD=2. 88% ) for doxofylline and 91. 10%( RSD=2. 56%) for methylprednisolone. Conclusion This method can simultaneously determine the serum concentrations of dox-ofylline and methylprednisolone, which is convenient, sensitive, accurate and can be used to study the pharmacokinetic interaction and monitoring of serum drug concentration.关键词
反相高效液相色谱法/多索茶碱/甲泼尼龙/血药浓度Key words
RP-HPLC/doxofylline/methylprednisolone/blood drug concentration分类
医药卫生引用本文复制引用
陈菲,王建美,盛柳青,裘颖儿..反相高效液相色谱法同时测定大鼠血清中多索茶碱和甲泼尼龙的质量浓度[J].中国药业,2015,(9):18-19,20,3.基金项目
浙江省医药卫生科技计划项目，项目编号2014KYA218。 （）
