中国药业Issue(9)：51-52,2.
高效液相色谱法测定止咳定喘丸中苦杏仁苷含量
Determination of Amygdalin Content in Zhike Dingchuan Pills by HPLC
杨慈海 1刘冬梅 1钟戈锐2
作者信息
- 1. 湖北省荆门市食品药品监督检验所，湖北 荆门 448124
- 2. 湖北百科亨迪药业有限公司，湖北 荆门 448000
- 折叠
摘要
Abstract
Objective To establish a HPLC method for the determination of the amygdalin content in Zhike Dingchuan Pills. Methods The Agela Venusil MP C18 column ( 250 mm × 4. 6 mm, 5 μm ) was used with the mobile phase of methanol-water ( 20 :80 ) , the flow rate was 1. 0 mL/min, the detection wavelength was 210 nm and the column temperature was the room temperature. Results The sam-ple size of amygdalin in the range of 0. 053 0-0. 848 0 μg ( r=0. 999 9 ) showed a good linear relationship with the peak area. The average recovery rate was 98. 64% with RSD=1. 10% ( n=6 ) . Conclusion This method is simple, sensitive and accurate, and can be used for the determination of the amygdalin content in Zhike Dingchuan Pills.关键词
高效液相色谱法/止咳定喘丸/苦杏仁苷/含量测定Key words
HPLC/Zhike Dingchuan Pills/amygdalin/content determination分类
医药卫生引用本文复制引用
杨慈海,刘冬梅,钟戈锐..高效液相色谱法测定止咳定喘丸中苦杏仁苷含量[J].中国药业,2015,(9):51-52,2.
