中国药业Issue(10)：23-25,26,4.
促红细胞生成素与甲基强的松龙对星形胶质细胞的作用研究
Effect of Erythropoietin and Methylprednisolone on Astrocytes
郝彦明 1王洪震 1贾正平 1徐又佳 2俞晨2
作者信息
- 1. 江苏省昆山市第一人民医院关节科，江苏 苏州 215300
- 2. 苏州大学附属第二医院骨科，江苏 苏州 215004
- 折叠
摘要
Abstract
Objective To study the effect of methylprednisolone(MPSS) combined with erythropoietin(EPO) to in vitro cultured primary astrocytes(AST). Methods The cell suspension was prepared by using the cerebrum of neonatal rats within 3 d of birth,cultured by the DMEM culture solution containing 15% fetal calf serum,gradually passaged after shaking for purifying astrocytes. After simulating nulli-nutrition model for 3 h by phosphate buffer solution(PBS) for substituting medium,MPSS 10 μmol/L,EPO 10 U/L and MPSS 10 U/L plus EPO 10 U/L were immediately added and the continuous culture for 3 d was performed. Astrocytes were identified by the immunofluorescent technique;the proliferation activity and apoptosis of astrocytes were detected by MTT;the expression level of GFAP mRNA was detected by PCR. Results Astrocytes were passaged to the third generation after shaking,the astrocytes purity reached more than 95% by identification;part of astrocytes became round in morphology and even died after nulli-nutrition for 3 h;after 3 d of applying MPSS,EPO and MPSS plus EPO in the normal group and the damage group,both cytoactive and expression level of GFAP mRNA in the damage group were significantly increased ( P ﹤ 0. 05 ) ,moreover compare with the single application of MPSS or EPO,the cytoactive and GFAP mRNA expression level in the combined application of MPSS and EPO were significantly increased( P ﹤ 0. 05),but no obvious change was found in the normal group( P ﹥ 0. 05). Conclusion The combined application of proper dose of MPSS and EPO has obviously protection effect on nulli-nutrition damaged astrocytes,moreover has significant difference compared with the single appli-cation of MPSS or EPO,but has no obvious protective effect on normal astrocytes.关键词
甲基强的松龙/促红细胞生成素/星形胶质细胞/缺营养/联合用药Key words
methylprednisolone/erythropoietin/astrocytes/nulli-nutrition/combined medication分类
医药卫生引用本文复制引用
郝彦明,王洪震,贾正平,徐又佳,俞晨..促红细胞生成素与甲基强的松龙对星形胶质细胞的作用研究[J].中国药业,2015,(10):23-25,26,4.
