中国药业Issue(10)：26-28,3.
反相高效液相色谱法测定去甲斑蝥素齐墩果酸复合脂质体包封率
RP-HPLC Determination of Entrapment Efficiency of Norcantharidin and Oleanolic Acid Complex Liposome
摘要
Abstract
Objective To establish a reversed-phase high performance liquid chromatography(RP-HPLC) method for the determination of entrapment efficiency of norcantharidin and oleanolic acid complex liposome. Methods The separation was achieved by using the Thermo Sycronis C18 column ( 150 mm × 4. 6 mm,5 μm ) with the gradient elution. The detection wavelength was set at 210 nm and the column temperature was 30 ℃. The free drugs in liposome were separated by using the upercentrifugation. Results The mass concentra-tion of norcantharidin in the range of 20-300 μg/mL showed the good linear relation with the peak area and the average recovery was 99. 15% -100. 11%,RSD ≤ 3. 20%( n=5);the mass concentration of oleanolic acid in the range of 10-150 μg/mL showed the good linear relation with the peak area and the average recovery was 99. 22% -100. 30%,RSD≤3. 34%( n=5). Conclusion The method is simple,fast,and thus can be applied to the determination of entrapment efficiency of norcantharidin and oleanolic acid com-plex liposome.关键词
反相高效液相色谱法/脂质体/去甲斑蝥素/齐墩果酸Key words
RP-HPLC/liposome/norcantharidin/oleanolic acid分类
化学化工引用本文复制引用
方志娥,陈薇,唐直婕,冷静..反相高效液相色谱法测定去甲斑蝥素齐墩果酸复合脂质体包封率[J].中国药业,2015,(10):26-28,3.基金项目
重庆市自然科学基金项目，项目编号CSTC，2010BB5347；重庆市卫生局中医药科技项目，项目编号2011-2-161。 （）
