摘要
Abstract
Objective To study the mice immunoregulation effect of the combined use of yellow propolis obtained by supercritical CO2 fluid extraction and black propolis obtained by the slag alcohol extraction. Methods Totally 160 SPF grade Kunming female mice were equally divided into 4 groups,40 cases in each group,The yellow and black propolis groups were gavaged by 3 doses of 0. 167,0. 333, 0. 450 g/(kg·bw)respectively,while the control group was gavaged by isometric soybean oil,lasting for 30 d. The mice's NK cell activity,ratio of immune organs and body weight,delayed-type hypersensitivity(DTH),ConA induced lymphocyte transformation ability, number of antibody generating cells,half value of hemolysin( HC50),phagocytosis index of macrophage phagocytosing cock's RBC and monocyte-macrophage carbon clearance were measured. Results The dose of 0. 450 g/(kg·bw)could significantly increase the DTH ability,ConA induced lymphocyte transformation ability,serum HC50 value and number of antibody generating cells( P ﹤ 0. 05). The doses of 0. 333 g/(kg·bw) and 0. 450 g/(kg·bw)yellow and black propolis could significantly increase the activity of NK cell(P ﹤ 0. 05). Yellow and black propolis obtained by supercritical CO2 fluid extraction had no obvious influence on the ratio of thymus and body weight, ratio of spleen and body weight,monocyte-macrophage carbon clearance ability cornbined with capacity of mice macrophage phagocytosing cock's RBC( P ﹥0. 05). Conclusion Yellow proplis combined with black propolis has the effect for increasing the immunoregulation function.关键词
蜂胶/超临界CO2流体萃取/免疫调节Key words
propolis/supercritical CO2 fluid extraction/immunoregulation分类
医药卫生
