中国药业Issue(10)：60-61,62,3.
高效液相色谱法测定四神片中吴茱萸碱和吴茱萸次碱含量
Determination of Evodiamine and Rutaecarpine Contents in Sishen Tablets by HPLC
潘瑞玲 1韩慧琴2
作者信息
- 1. 内蒙古医学院第二附属医院，内蒙古 呼和浩特 010030
- 2. 河北省张家口市食品药品检验中心，河北 张家口 075000
- 折叠
摘要
Abstract
Objective To establish a HPLC method for determining the evodiamine and rutaecarpine contents in Sishen Tablets. Methods The Waters 2695-2996 type HPLC was adopted with Capcell PAK-C18 column(150 mm × 4. 6 mm,5 μm)as fixed phase and 0. 1%phosphoric acid solution(containing 0. 2% sodium dodecylsulfate)-methanol-acetonitrile(50 :20 :30)as the mobile phase. The detec-tive wave length was 225 nm for evodiamine and 343 nm for rutaecarpine. The flow rate was 1. 0 mL/min. Results The linearity range of evodiamine was 0. 003 8-0. 750 0 μg( r=0. 999 9),the average recovery rate was 100. 19%,RSD=0. 18%( n=6);the lin-earity range of rutaecarpine was 0. 002 5-0. 500 0 μg( r=0. 999 9),the average recovery rate was 100. 10%,RSD=0. 44%( n=6). Conclusion The method is simple,accurate,better reproducible and suitable for the quantitative determination of evodiamine and rutae-carpine contents in Sishen Tablets.关键词
四神片/吴茱萸碱/吴茱萸次碱/高效液相色谱法/含量测定Key words
Sishen Tablet/evodiamine/rutaecarpine/HPLC/content determination分类
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潘瑞玲,韩慧琴..高效液相色谱法测定四神片中吴茱萸碱和吴茱萸次碱含量[J].中国药业,2015,(10):60-61,62,3.
