摘要
Abstract
Objective To establish a HPLC method for the determination of ochratoxin A(OTA)contamination in the Chinese medicinal ma-terials. Methods The powdered medicinal materials were extracted by methanol-water(80 :20),purified by the OTA immunoaffinity column and determined. The chromatographic column was with the Agilent Zorbax SB-C18 column(250 mm × 4. 60 mm,5 μm),the mobile phase was acetonitrile-water-acetic acid(49. 5 :49. 5 :1. 0),the flow rate was 1 mL/min,detected by the fluorescence detector,the excitation wave-length was 333 nm and the emission wavelength was 460 nm,the column temperature was 35 ℃,the sample temperature was 10 ℃ and the sample volume was 20 μL. Results The area of chromatographic peak and the concentration demonstrated a good linear relationship,its de-tection limit was 0. 2 ng/g,the recovery rate of samples was 64. 4% -106%,RSD was 0-28. 5%( n=3). Conclusion This method is sim-ple,accurate,better reproducible,which can be used for the determination of OTA in the Chinese medicinal materials.关键词
中药材/赭曲霉毒素A/高效液相色谱法/免疫亲和柱Key words
Chinese medicinal materials/ochratoxin A/HPLC/immunoaffinity column分类
医药卫生
