中国药业Issue(13)：33-34,2.
高效液相色谱－质谱联用法检测抗鼻炎喷剂中非法添加盐酸萘甲唑林
Determination of Naphazoline Hydrochloride Illegally Added in Anti-Rhinitis Spray by LC-MS/MS
摘要
Abstract
Objective To develop a LC-MS/MS method for the determination of illegally added chemical substance naphazoline hy-drochloride in anti-rhinitis spray. Methods LC-MS/MS method was adopted. The separation was achieved by using the Zorbax Eclipse XDB-C18 column ( 150 mm × 4. 6 mm, 5 μm ) and the mobile phase of acetonitrile-3% acetic acid-triethylamine ( 15 :85 :0. 08, adjusting pH=3. 0 with acetic acid or triethylamine ) . The flow rate was 0. 2 mL/min and the column temperature was set at 35℃. The electrospray ionization was adopted. The parent ion ( m/ z 210 ) and daughter ion ( m/ z 140, 115 ) of naphazoline hydrochlo-ride were detected by the multiple-reaction monitoring scanning mode via positive ion mode. Results The limit of detection ( LOD ) for illegally added naphazoline hydrochloride in anti-rhinitis spray was below 0. 05 ng. Among the 24 batches of commercially available anti-rhinitis spray, naphazoline hydrochloride was detected out in 17 batches. Conclusion This established method is strongly specific and highly sensitive, and can be used for the screening and confirmation work of illegally added naphazoline hydrochloride in anti-rhinitis spray.关键词
高效液相色谱-质谱/抗鼻炎喷剂/非法添加/盐酸萘甲唑林Key words
LC-MS/MS/anti-rhinitis spray/illegal addition/naphazoline hydrochloride分类
医药卫生引用本文复制引用
陈日檬,柯春文,万晓明,李德良,李蔚,谢立宇..高效液相色谱－质谱联用法检测抗鼻炎喷剂中非法添加盐酸萘甲唑林[J].中国药业,2015,(13):33-34,2.基金项目
广东省药检系统非法添加系统技术策略专项资助课题，项目编号YJ201074。 （）
