中国医药科学Issue(6)：142-144,3.
TPPA和酶联免疫吸附法在梅毒诊断中的作用
Role of TPPA and enzyme linked immunosorbent assay in diagnosis of syphilis
摘要
Abstract
Objective To compare and analyze the differences between TPPA and enzyme linked immunosorbent assay in the diagnosis of syphilis and to explore their advantages. Methods 260 patients treated in the STD outpatient of our center from January 2011 to October 2013 were selected as the study subjects and randomly divided into the experimental group and the control group, with 130 patients in each group. The experimental group used TPPA to detect Treponema pallidum antibodies and the control group used enzyme linked immunosorbent assay to detect Treponema pallidum antibodies. Results In the experimental group, the positive rate of TPPA detection of Treponema pallidum antibodies was 26.92%(35/130); In the control group, the positive rate of TPPA detection of Treponema pallidum antibodies was 20.00%(26/130). If the enzyme linked immunosorbent assay served as the gold diagnostic standards, the sensitivity and specificity of TPPA were 93.65% and 97.68% respectively. Conclusion In terms of sensitivity and specificity of Treponema pallidum antibodies detection, TPPA has better specificity than enzyme linked immunosorbent assay, but enzyme linked immunosorbent assay has higher sensitivity than TPPA. In clinical practice, enzyme linked immunosorbent assay should serve as the screening test of syphilis, and for the screened positive samples, TPPA should be used to confirm the diagnosis. Only by effective combination of the two methods, misdiagnosis and missed diagnosis can be avoided, and the combined method has the advantages of good repeatability, thereby worthy of clinical application and promotion.关键词
梅毒/TPPA/酶联免疫吸附法Key words
Syphilis/TPPA/Enzyme linked immunosorbent assay分类
医药卫生引用本文复制引用
刘春华,孙国清,薛秀娟,王哲..TPPA和酶联免疫吸附法在梅毒诊断中的作用[J].中国医药科学,2014,(6):142-144,3.基金项目
国家十二五科技重大专项（2012ZX10004-905）。 （）
