摘要
Abstract
To investigate the effects of alendronate combined with simvastatin on the osteoporotic fracture healing in rats.Methods 50 SD adult female rats were randomly divided into 5 groups:(A)sham operation control group;(B)ovariectomized control group;(C)ovariectomized alendronate group;(D)simvastatin group;(E) ovariectomized alendronate and simvastatin group.B,C,D,Egroup underwent ovariectomy surgery,A group only partially excised adipose tissue.8 weeks later,2 rats were selected randomly from each group to confirm osteoporosis successful modeling.Then the midshaft femur fracture model was established by an operation and intramedullary nails fixation was achieved with Kirschner wire.Deal each group of rats with such drug intervention as follows:(A,B) physiological saline 5mL/(kg·d)gavage for 8 weeks; (C)alendronate 0.5mg/(kg·d) gavage for 8 weeks;(D) simvastatin 20mg/(kg·d)gavage for 8 weeks (E)alendronate 0.5mg/(kg·d)+ simvastatin 20mg/(kg·d) gavage for 8 weeks.All the rats were killed and their blood samples and left femurs were collected.The serum calcium, phosphorus,alkaline phosphatase(ALP)content,X-ray bone mineral density(BMD) and biomechanical strength were detected.Results Compared with the sham group,the ovariectomized group had significantly reduced bone mineral density,elevated ALP level and serum calcium,phosphorus content had no significant change.After treatment witn alendronate and simvastatin for 8 weeks,calcium,phosphorus content had no significant change;alkaline phosphatase level decreased;femoral bone mineral density and biomechanical strength were improved.This result is more obvious in alendronate and simvastatin group, the difference was statistically significant.Conclusion Alendronate is beneficial to the restoration of biomechanical function in the osteoporosis fracture healing,and simvastatin can play a synergistic effect.关键词
双膦酸盐/阿仑膦酸钠/辛伐他汀/骨质疏松/骨折Key words
Bisphosphonates/Alendronate/Simvastatin/Osteoporosis/Fracture healing分类
医药卫生
