中国医药科学Issue(12)：11-13,3.
阿霉素加热化疗对人肝癌细胞HHCC及HepG2的抑制作用研究
Inhibiting effect of doxorubicin heating chemotherapy in proliferation and apoptosis of hepatic carcinoma HHCC and HepG2 cells
摘要
Abstract
Objective To investigate the inhibiting effect of doxorubicin heating chemotherapy in proliferation of hepatic carcinoma HHCC and HepG2 cells. Methods Invitro culture HHCC and HepG2 cells in logarithmic growth phase were selected for the study,10%fetal bovine serum medium was used to formulated single cell suspension with cell density of 5×104/mL,and then inoculated in the 50mL cultivation bottle. The cells were divided into 3 groups,and given simple heating, simple chemotherapy,heating chemotherapy treatment respectively,cell proliferation was detected by MTT assay,and was apoptosis rate was detected by flow cytometer. Results Proliferation inhibition rate of HHCC and HepG2 cell among the three groups compared,the difference was statistically significant(P < 0.05), inhibition rate of heating chemotherapy group was significantly higher than simple heating group and simple chemotherapy group (P < 0.05);apoptosis rate of HHCC and HepG2 cells of three groups compared,differences was statistically significant (P < 0.05).apoptosis rate of heating chemotherapy group was significantly higher than simple heating group and simple chemotherapy group (P<0.05). Conclusion Doxorubicin heating chemotherapy can significantly enhance inhibiting effect of doxorubicin on HHCC and HepG2 hepatoma cell, it has reference significance for clinical treatment .关键词
阿霉素/热化疗/人肝癌细胞/细胞增殖Key words
Doxorubicin/Heating chemotherapy/Human hepatoma cells/Cell proliferation分类
医药卫生引用本文复制引用
侯敬申,侯敬侠,王伟雄,赵莉..阿霉素加热化疗对人肝癌细胞HHCC及HepG2的抑制作用研究[J].中国医药科学,2014,(12):11-13,3.基金项目
国家自然科学基金青年项目（81302357）；广东省自然科学基金博士启动项目（S2013040016493）；广东高校优秀青年创新人才培养计划项目育苗工程（自然科学）（2013LYM0075）。 （）
