中国医药科学Issue(14)：88-90,3.
HPLC法测定超微戊己丸中吴茱萸碱和吴茱萸次碱含量
Measurement of evodiamine content and rutaecarpin content in ultramicro Wuji pills based on HPLC method
摘要
Abstract
Objective To establish a method for measurement of evodiamine content and rutaecarpin content in ultramicro Wuji pills. Methods Chromatographic column: Kromasail C18(250mm×4.6mm, 5μm); mobile phase:acetonitrile-acetonitrile(50:50); flow rate: 1.0mL/min; wavelength: 225nm. Results The number of theoretical plates for evodiamine and rutaecarpin was 2681 and 2067 respectively(Yevodiamine=1.965×10-7X+0.07646, r=0.9999;Yrutaecarpin=3.658×10-7X-0.2199, r=0.9999). Evodiamine and rutaecarpin had linear interval of 10.2-51.0μg/mL and 10.0-50.0μg/mL respectively, average recovery rate of 97.3% and 101.4% respectively, relative standard deviation of 3.2% and 3.9% respectively, lowest detection limit of 0.05μg/mL and 0.1μg/mL respectively. Conclusion High performance liquid chromatography demonstrates high accuracy and good reproducibility for measurement of evodiamine content and rutaecarpin content in ultramicro Wuji pills.关键词
HPLC/吴茱萸碱/吴茱萸次碱Key words
HPLC/Evodiamine/Rutaecarpin分类
医药卫生引用本文复制引用
江星明,李荣东..HPLC法测定超微戊己丸中吴茱萸碱和吴茱萸次碱含量[J].中国医药科学,2014,(14):88-90,3.基金项目
湖南省教育厅科学研究项目（13C675）。 （）
