中国医药科学Issue(17)：106-109,117,5.
UPLC-MS/MS法快速检测减肥类保健食品中5种非法添加化学成分
Rapid identification of 5 illegal additives in slimming health food by UPLC-MS/MS
摘要
Abstract
Objective The study aimed to develop a specific, sensitive and rapid UPLC-MS/MS method to identify 5 illegal additives in slimming health food.MethodsThe ultra performance liquid chromatography-electrospray ion trap mass spectrometry method was used to detect the samples extracted by methanol. The separation was performed on Waters UPLC using BEH-C18 column with a gradient elution. The mobile phase was consisted of 20mmol/L ammonium acetate (containing 0.1% acetic acid) and methanol. Qualitative and quantitative analysis of caffeine, furosemide, fenfluramine, phenolphthalein and sibutramine was conducted in the MRM mode by UPLC-MS/MS.ResultsA good resolution of 5 chemical drugs within 6 min was obtained by UPLC-MS/MS. The calibration curves showed good linear relationship in the range of 1-200ng/mL for each component. The determination limits was at 0.0025-0.0075mg/g, and the average recovery rate was 97.7%-100.7%. Conclusion The method was proved to be specific, rapid and sensitive, which was able to be used to identify 5 illegal additives in weight reducing health food.关键词
UPLC-MS/MS/MRM/非法添加Key words
UPLC-MS/MS/MRM/Illegal additives分类
轻工纺织引用本文复制引用
郝刚,顾炳仁..UPLC-MS/MS法快速检测减肥类保健食品中5种非法添加化学成分[J].中国医药科学,2014,(17):106-109,117,5.基金项目
江苏省苏州市科技发展计划项目科技支撑计划（SS201220）。 （）
