中国医药科学Issue(20)：7-12,26,7.
黄芪山甲方抗肾间质纤维化的多靶点抑制作用
Multi-target inhibition of Huangqishanjia (HQSJ) on renal interstitial fibrosis(RIF)
摘要
Abstract
Objective To observe the multi-target therapeutic effect of HQSJ for RIF of mice and discuss possible action mechanism of HQSJ in different stages. Methods UUO mice mode was adopted and 54 male SD mice were randomly divided into 3 groups as fake operation group (Sham), model group (UUO) and treatment group (HQSJ). 6 mice were killed separately on 14d, 28d and 56d after operation and their kidney function changes were observed;TBM, renal tubular atrophy, inflammatory cell infiltration and RIF were given semi-quantitative scores;TGF-β1, FN protein and mRNA expressions of renal tissues were detected by immunohistochemical method and RT-PCR method. Results (1) On kidney function, compared with Sham group, BUN and SCr values of UUO and HQSJ group were developed during 14-28d and stayed relatively steady during 28-56d. BUN and SCr values of HQSJ group were both lower than UUO group in the same time phase (P < 0.05). (2) On pathological chages, compared with Sham group, inflammatory cell infiltration and renal interstitial chronic lesion degrees of mice of UUO and HQSJ group both had a increasing trend in the same time phase (P<0.05). There were significant differences between inflammatory cell infiltration of UUO group and HQSJ group on 14 and 28d (P < 0.05) but no significant difference on 56d (P > 0.05); there were significant differences on renal interstitial chronic lesion degrees on 14, 28 and 56d (P<0.05). (3) On TGF-β1, FN expressions:compared with Sham group, TGF-β1 and FN expressions of UUO and HQSJ group both rose in the same time phase (P<0.05); compared with UUO group, TGF-β1 and FN expressions on 14, 28 and 56d of HQSJ group all declined to different extents (P<0.05);decline degree of the same time phase comparison;immunohistochemical method showed that positive rates of TGF-β1 and FN protein declined separately by 64%, 47%, 7% and 82%, 72%, 70%; RT-PCR method showed that mRNA of TGF-β1 and FN declined separately by 72%, 57%, 27%%and 83%, 72%, 68%. Conclusion Multi-target therapeutic effect of HQSJ for RIF may related with decrease of ECM deposition caused by decrease of inflammatory cells, inhibition of TGF-β1 secretion, alleviation of excitation and transdifferentiation of renal inherent cells. Its action started from early lesion, mainly reducing inflammatory cell infiltration and decreasing TGF-β1 expression, and inhibition to FN throughout the whole process.关键词
黄芪山甲方/肾间质纤维化/TGF-β1/FNKey words
Huangqishanjia/Renal interstitial fibrosis/TGF-β1/FN分类
医药卫生引用本文复制引用
蔡宏瑜,杨光辉,万强,张娟,傅志慧,高艳霞..黄芪山甲方抗肾间质纤维化的多靶点抑制作用[J].中国医药科学,2014,(20):7-12,26,7.基金项目
山西省基础研究项目（2011011039-2）。 （）
