中国医药科学Issue(20)：54-57,82,5.
HPLC法测定葛根总黄酮固体脂质纳米粒的载药量及包封率
Determination of drug loading and entrapment efficiency of pueraria flavonoids loaded solid lipid nanoparticles by HPLC
摘要
Abstract
Objective To establish a method for simultaneous determination of drug loading and entrapment efficiency of four isoflavones in pueraria flavonoids (PF)loaded solid lipid nanoparticles(PF-SLN). Methods HPLC determination was performed on a Kromasil C18 column (4.6mm×250mm, 5μm) and detected at 250 nm. The mobile phase was consisted of methanol and 0.1% citric acid solution with gradient elution. Flow-rate was 1.0mL/min, and column temperature was 40℃. The free drugs in solid lipid nanoparticles were separated by high-speed centrifuge. Results 3’-hydroxypuerarin, puerarin, daidzin and daidzein had a good linear relation and the average recoveries were (100.28±2.52)%, (100.26±2.33)%, (100.08±3.35)% and (100.44±3.48)%, respectively. The entrapment efficiency of 3’-hydroxypuerarin, puerarin, daidzin and daidzein in PF-SLNs were (84.35±0.45)%, (86.84±0.48)%, (89.52±0.86)% and (93.80±0.50)%, and drug loading were (10.37±0.36)%, (14.19±0.52)%, (16.79±0.34)% and (20.00±0.97)%, respectively. Conclusion Results obtained showed that it’s a convenient, accurate and reliable method. The method has been successfully applied to determination of drug loading and entrapment efficiency of four isoflavonoids in PF-SLNs.关键词
葛根总黄酮/固体脂质纳米粒/载药量/包封率Key words
Pueraria flavonoids/Solid lipid nanoparticles/Drug loading/Entrapment efficiency分类
化学化工引用本文复制引用
熊月路,崔蕴慧,曾天骏,巫嘉华,李冬玲,王治平,王一飞..HPLC法测定葛根总黄酮固体脂质纳米粒的载药量及包封率[J].中国医药科学,2014,(20):54-57,82,5.基金项目
广东省科技计划项目（2012A080204003）。 （）
