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裂褶菌子实体中多糖及蛋白质含量的测定

孙鹏珍 刘月 王大可 李爱欣 王淑敏

中国医药科学Issue(23):74-76,210,4.
中国医药科学Issue(23):74-76,210,4.

裂褶菌子实体中多糖及蛋白质含量的测定

Content determination of polysaccharide and protein in Schizophyllum commune sporophore

孙鹏珍 1刘月 1王大可 1李爱欣 1王淑敏1

作者信息

  • 1. 长春中医药大学药学院,吉林长春130117
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摘要

Abstract

Objective Set up Schizophyllumcommune sporophore polysaccharide and protein content determination method. Methods Use spectrophotometry to determine polysaccharide and protein content, detection wavelength is 479nm and 595nm. Results Polysaccharide concentration possesses good linear relationship between 0.012-0.010mg/mL, regression equation is Y=23.0753X-0.1733, r=0.9998, sporophore polysaccharide content is 7.26%; protein concentration possesses good linear relationship between 0.0078-0.0321mg/mL, regression equation is Y=20.0830X+0.1451, r=0.9997, sporophore total protein is 0.2631%. Conclusion The method set up by the research is simple, fast, accurate and has good repeatability which provides basis for Schizophyllumcommune sporophore polysaccharide and protein content determination.

关键词

裂褶菌/多糖/蛋白质/含量测定

Key words

Schizophyllumcommune/Polysaccharide/Protein/Content determination

分类

化学化工

引用本文复制引用

孙鹏珍,刘月,王大可,李爱欣,王淑敏..裂褶菌子实体中多糖及蛋白质含量的测定[J].中国医药科学,2014,(23):74-76,210,4.

基金项目

长春中医药大学大学生“SRT”项目(201310199051)。 ()

中国医药科学

2095-0616

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