中国医药科学Issue(23):74-76,210,4.
裂褶菌子实体中多糖及蛋白质含量的测定
Content determination of polysaccharide and protein in Schizophyllum commune sporophore
摘要
Abstract
Objective Set up Schizophyllumcommune sporophore polysaccharide and protein content determination method. Methods Use spectrophotometry to determine polysaccharide and protein content, detection wavelength is 479nm and 595nm. Results Polysaccharide concentration possesses good linear relationship between 0.012-0.010mg/mL, regression equation is Y=23.0753X-0.1733, r=0.9998, sporophore polysaccharide content is 7.26%; protein concentration possesses good linear relationship between 0.0078-0.0321mg/mL, regression equation is Y=20.0830X+0.1451, r=0.9997, sporophore total protein is 0.2631%. Conclusion The method set up by the research is simple, fast, accurate and has good repeatability which provides basis for Schizophyllumcommune sporophore polysaccharide and protein content determination.关键词
裂褶菌/多糖/蛋白质/含量测定Key words
Schizophyllumcommune/Polysaccharide/Protein/Content determination分类
化学化工引用本文复制引用
孙鹏珍,刘月,王大可,李爱欣,王淑敏..裂褶菌子实体中多糖及蛋白质含量的测定[J].中国医药科学,2014,(23):74-76,210,4.基金项目
长春中医药大学大学生“SRT”项目(201310199051)。 ()