中国医药科学Issue(3)：54-56,3.
高效液相色谱法测定人血浆中依托咪酯的浓度
Determination of etomidate concentration in plasma by HPLC
张海峰 1马爱玲 1王漪檬 1修佳 1赵红卫 1秦玉花 1赵宁民1
作者信息
- 1. 河南省人民医院药学部，河南郑州450003
- 折叠
摘要
Abstract
Objective To develop a method for the determination of etomidate concentration in plasma by using HPLC and to provide scientific analysis for the studies of etomidate clinical pharmacology. Methods The Agilent TC-C18 (250mm×4.6mm,5μm) was used as analytical columu with mobile phase consisted of methanol and water (75:25) .The flow rate was 1.0 mL/min and detection wavelength was 241nm. Results The linear ranges were 0.25-5.00μg/mL for etomidate in human plasma.The method recover were 98.6%-101.3%and the extraction recovery were 72.6%-74.6%. Conclusion The method is simple, accurate and reliable which is suitable for the determination of etomidate in human plasma.关键词
依托咪酯/人血浆/高效液相色谱法Key words
Etomidate/Human plasma/HPLC分类
医药卫生引用本文复制引用
张海峰,马爱玲,王漪檬,修佳,赵红卫,秦玉花,赵宁民..高效液相色谱法测定人血浆中依托咪酯的浓度[J].中国医药科学,2015,(3):54-56,3.
