摘要
Abstract
ObjectiveTo establish an method for the determination of Oseltamivir Phosphate capsules.Methods HPLC method was adopted.The separation was performed on Kromasil C8(250mm×4.6mm,5μm), column with mobile phase consisted of 0.05mol/L postassiam dihydrogen phosphate (adjust pH to 5.5 with 40% potassium hydroxide)- methanol- acetonitrile(70:20:10). UV detection wavelength was set at 207nm,the column temperature was maintained at 30℃,the flow rate was 1.0mL/min and the sample size was 20μL.Results The line arrange of Oseltamivir Phosphate was 1-50μg,the correlation coefficient was 0.9990 and the average recovery was 99.8%(RSD=0.9%,n=9).RSDs of stability test,precision test,reproducibility test were all less than 0.8%. Conclusion The method is simple,quick, accurate and suitable for the content determination of Oseltamivir Phosphate capsules.关键词
高相液相色谱法/磷酸奥司他韦/含量测定Key words
HPLC/Oseltamivir phosphate/Content determination分类
医药卫生
