中国真菌学杂志Issue(5)：264-267,4.
不同毒力差异基因型的新生隐球菌MLST分型及交配型鉴定研究
MLST genotyping and mating type identification of C. neoformans in genotypes with virulent difference
摘要
Abstract
Objective To study the Multilocus Sequence Typing( MLST)characteristics of C. neoformans var grubii and to i-dentify their mating types Methods Primers of 7 housekeeping genes(CAP59,GPD1,LAC1,PLB1,SOD1,URA5 and IGS1)were designed for PCR amplification;the genotypes of 20 environment and clinical isolates were analyzed with MLST result and the mat-ting types of these strains were identified And both the results of MLST and Multilocus Microsatellite Typing( MLMT)were com-pared to each other for evaluating their stability and reliability in taxonomy Results The multilocus microsatellite genotype of all the 10 environment isolates was MLMT-13 in previous experiment and the MLST type of there strains was ST-15 in this experiment Sim-ilarly,all the MLMT-36 clinical isolates belong to ST-32 And all the strains were MAT-α Conclusion The MLST results are high-ly consistent with MLMT results in previous experiment which reveals that both molecular typing methods are high resoluted and sta-ble on classification of different isolation background on species or varieties level.关键词
新生隐球菌/MLST分型/MLMT分型/交配型鉴定Key words
Cryptococcus neoformance/MLST/MLMT/mating type分类
医药卫生引用本文复制引用
康颖倩,陈玉如,王梅竹,赵亮,李小玲,金方,刘涛华..不同毒力差异基因型的新生隐球菌MLST分型及交配型鉴定研究[J].中国真菌学杂志,2014,(5):264-267,4.基金项目
国家自然基金地方项目（31060006，31260029）；贵州省科技厅国际合作项目（黔科合外G字［2009］700114号）；贵州省社会发展科技攻关项目（黔科合SY字［2011］3017号）；贵阳市科技局社会发展与民生计划 （）
