中国真菌学杂志Issue(1)：25-29,30,6.
白头翁汤正丁醇提取物对白念珠菌VVC临床株黏附作用的影响
Anti-adhesion effect of Butyl alcohol extract of BaiTouWeng decoction on Candida albicans isolated from VVC
摘要
Abstract
Objective To investigate anti⁃adhesion effect of Butyl alcohol extract of BaiTouWeng decoction ( BAEB) on Candida albicans.Methods Agar plate assay was used to determine CFU of Candida albicans for 2h and 4h.XTT reduction assay was applied to evaluate the adherent Candida albicans merabolic activity.Test tube assay was utilized to evaluate flocculation.The water⁃hydrocar⁃bon two⁃phase assay was employed to measure the cell surface hydrophobicity of Candida albicans . Scanning electron microscope (SEM)and fluorescein diacetate(FDA)staining was used to observe the morphological changes of adherent Candida albicans .qRT⁃PCR was adopted to inspect the expression of adhesion⁃related genes such as INT1, ALS3, MNT2, ALA1, ALS1, ALS5, HWP1 and CSH1.Results 512,1 024μg/mL BAEB could effectively inhibit adherence of Candida albicans .Flocculation was delayed by 512, 1 024 μg/mL BAEB.The cell surface hydrophobicity in the BAEB treated groups was lower than that of the control group.The expres⁃sion of ALS1,CSH1,ALA1,ALS3,INT1,HWP1 were downregulated dramatically after BAEB treatment,but ALS5 and MNT2 were un⁃changed.Conclusion BAEB could effectively inhibit adherence of Candida albicans isolated from VVC by regulating adhesion⁃asso⁃ciated genes.关键词
白头翁汤正丁醇提取物/外阴阴道念珠菌病/白念珠菌/黏附Key words
butyl alcohol extract of BaiTouWeng decoction ( BAEB)/vulvovaginal candidiasis( VVC)/Candida albicans/adhe-sion分类
医药卫生引用本文复制引用
张梦翔,夏丹,施高翔,陆克乔,邵菁,吴大强,汪天明,汪长中..白头翁汤正丁醇提取物对白念珠菌VVC临床株黏附作用的影响[J].中国真菌学杂志,2015,(1):25-29,30,6.基金项目
安徽省自然科学基金（1408085MH165），安徽中医药大学校级科研项目 （）
