中国真菌学杂志Issue(2)：75-79,5.
转录因子 RPN4对白念珠菌药物敏感性的研究
Analysis of relationship between transcription factor RPN4 and drug susceptibility of Candida albicans
摘要
Abstract
Objective To study the influence on the drug susceptibility of Candida albicans after transcription factor RPN4 was knocked out and the mechanism how this transcription factor regulates the drug susceptibility in Candida albicans.Methods The broth microdilution method was used to determine the minimal inhibitory concentrations (MICs) of 30 strains transcription factor knocked out Candida albicans .The drug susceptibility was reassured by spot assay and growth curve.The total RNA of parental strain SN250 and susceptible strains were extracted and the expression of multidrug resistance genes CDR1 and MDR1 was determined by real⁃time PCR,and the pump ability was examined by the ability of pump out rhodamine 6G.Results Compaired to the parental strain SN250,transcription factor RPN4 knocked out strain was more susceptible to fluconazole.The expression of CDR1 and MDR1 was lower than SN250 and the pump ability was also weaker than SN250.Conclusion The knockout of transcription factor PRN4 re⁃sults susceptible to fluconazole in Candida albicans,and this transcription factor may be involved in the regulation of drug susceptibil⁃ity in Candida albicans.关键词
白念珠菌/转录因子/药物敏感性/多药耐药基因Key words
Candida albicans/transcription factors/drug susceptibility/multidrug resistance genes分类
医药卫生引用本文复制引用
许洪涛,唐若愚,屈莺歌,王晓娟,姜远英,曹永兵,颜天华..转录因子 RPN4对白念珠菌药物敏感性的研究[J].中国真菌学杂志,2015,(2):75-79,5.基金项目
国家自然科学基金（81173100），上海市基础研究重点课题 （）
