中国真菌学杂志Issue(3)：139-145,7.
黄芩苷联合氟康唑对白念珠菌生物膜的抑制作用研究
Inhibitory effects of baicalin in combination with fluconazole against Candida albicans biofilms
摘要
Abstract
Objective This study aimed to investigate the antifungal activity of baicalin alone or in combination with fluconazole against Candida albicans biofilms and to explore the related mechanism.Methods Checkerboard method was uased to study the in⁃terrelation of baicalin and fluconazole on C.albicans;Time⁃kill curve was used to evaluate the effect of baicalin alone and in combina⁃tion with fluconazole on C.albicans;XTT reduction assay and measurement of biofilm biomass assay were performed to investigate the antibiofilm activity of baicalin alone and in combination with fluconazole;Scanning electron microscopy ( SEM ) and confocal laser scanning microscopy (CLSM) were used to observe the three⁃dimensional structure of C.albicans biofilms;Cellular surface hydropho⁃bicity ( CSH) assay was performed to investigate the effect of baicalin alone and in combination with fluconazole on adherence in C. albicans;Quantitative real time PCR (qRT⁃PCR) was performed to measure the expression of adhesion and hypal related genes.Re⁃sults Synergistic effect was showed in anti⁃biofilm by baicalin in combination with fluconazole,and FICI was between 0.28⁃0.5;SEM and CLSM showed that baicalin alone and in combination with fluconazole could destruct and resulted in scant or nonexistent bio⁃films;And expressions of ALS1,ALS3,EAP1,SUN41,CSH1 were significantly downregulated 6%,51%,24%,13%和39% by qRT⁃PCR.Conclusion Synergistic effect of baicalin in combination with fluconazole on C.albicans biofilm included by influenced the ex⁃pression of adhesion,CSH controlling genes,and decreased the CSH.关键词
黄芩苷/白念珠菌/生物膜/氟康唑/协同Key words
baicalin/C.albicans/biofilms/fluconazole/synergism分类
医药卫生引用本文复制引用
颜贵明,施高翔,邵菁,汪天明,夏丹,汪长中..黄芩苷联合氟康唑对白念珠菌生物膜的抑制作用研究[J].中国真菌学杂志,2015,(3):139-145,7.基金项目
安徽省自然科学基金（1408085MH165），安徽中医药大学校级科研项目 （）
