中国中西医结合外科杂志Issue(6)：607-611,5.DOI:10.3969/j.issn.1007-6948.2014.06.011
绿原酸对深静脉血栓形成大鼠T细胞亚群失衡的影响
Regulating Effect of Chlorogenic Acid on T cell Subsets Imbalance in Deep Venous Thrombosis of Rats
摘要
Abstract
Objective To evaluate the role of Th1/Th2 shift in deep venous thrombosis (DVT) and eluci⁃date the mechanism of chlorogenic acid in treating DVT in rat models. Method Forty DVT rats were divided randomly into DVT group and chlorogenic acid high dose, middle dose and low dose groups. Additionally, 10 rats with sham operation were taken as sham operation group and 10 normal rats were taken as normal group re⁃spectively. Rats of chlorogenic acid group were treated with chlorogenic acid for 7 days, while rats of other groups were treated with physiological saline for the same duration. Then, the proportions of Th1 (CD4+TNF-α+) subset and Th2 (CD4+IL-4+) subset were detected by flowcytometry. Moreover, the protein expressions of IL-2, TNF-α, IL-4 and IL-10 in rat serum were analyzed by ELISA assay, and the proportion of Th1/ Th2 correlated with serum levels of inflammatory markers was analyzed. Results Compared with normal and sham operation groups, the proportion of Th1 subset was increased while that of Th2 subset was decreased significantly in DVT group(22.71 ± 0.87%,3.06 ± 0.15%). In addition, pro⁃tein expression of IL-2 and TNF-α was enhanced (72.10 ± 7.48 173.50 ± 17.02, pg/mL)while that of IL-4 and IL-10 was inhibited markedly in DVT rats (347.46 ± 11.24 28.55 ± 1.78,pg/mL). After chlorogen⁃ic acid administration, the proportion of Th1 subset was down-regulated while that of Th2 subset was up-regulated significantly in DVT rats(14.38 ± 0.4 14.91 ± 0.14, %). Moreover, the protein expression of IL-2 and TNF-α decreased while that of IL-4 and IL-10 increased obviously after chlorogenic acid administra⁃tion, Th1/ Th2 proportional positively correlated with IL-2 and TNF-α, and negatively correlated with IL-4, IL-10(50.22 ± 4.06 110.22 ± 10.32, 418.72 ± 15.32 9.32 ± 2.83,pg/mL). Conclusion Th1/Th2 shift is involved in DVT. Chlorogenic acid can down-regulate the Th1 subsets and up-regulate the Th2 subsets and exert the cu⁃rative effect on DVT mice.关键词
绿原酸/Th1/Th2亚群/深静脉血栓形成/细胞因子Key words
Chlorogenic acid/Th1/Th2 subsets/deep vein thrombosis/cytokines分类
生物科学引用本文复制引用
刘礼青,王德华,王彬,李霞,田楠楠..绿原酸对深静脉血栓形成大鼠T细胞亚群失衡的影响[J].中国中西医结合外科杂志,2014,(6):607-611,5.基金项目
国家自然科学基金资助项目 （）
山东省优秀青年科学家奖励基金 （）
山东省中医药发展计划 （）
济南市科技明星计划项目 （）
