中国中西医结合外科杂志Issue(6)：611-614,4.DOI:10.3969/j.issn.1007-6948.2014.06.012
清胰颗粒对重症急性胰腺炎大鼠肠黏膜上皮紧密连接的保护作用
Effect of Qing Yi Particles on Intestinal Mucosal Tight Junction Protein Expression in Rats with Severe Acute Pancreatitis
赵光 1张淑坤 2崔乃强1
作者信息
- 1. 天津市南开医院肝胆胰外科一 天津 300100
- 2. 天津南开医院急腹症研究所 天津 300100
- 折叠
摘要
Abstract
Objective To investigate the effects of Qingyi particles on the intestinal mucosal tight junction protein in rats with severe acute pancreatitis (SAP). Methods Twenty-four healthy male Wistar rats were ran⁃domly divided into 3 groups: sham-operation group, SAP group and QingYi particle group. The SAP group and QingYi particle group were retrogradely cholangiopancreatographically injected with 3.5% sodium taurocholate to establish SAP model. QingYi particle group was given orally Qingyi particles at 2h before induction of SAP and 6h and 18h after induction of SAP. The SAP group was given placebo in the same times. At 24h,the small intestine tissue damage was examined by HE staining and Chiu evaluation standard, the expression of Occludin was detected by using Western blot and re⁃al-time quantitative PCR respectively. Results The ileum histopathological Chiu scores in the rats of SAP group were significantly higher than those in sham-operated group(4.6 ± 0.6 vs 0.0 ± 0.0),The ileum histopatho⁃logical Chiu scores in the Qingyi particle group were lower than those in the SAP group(2.0 ± 0.4vs4.6 ± 0.6);Compared to sham-operated group,the levels of Occludin protein(1.2±0.4vs3.2±0.4)and mRNA(1.3±0.3vs2.9± 0.5) in SAP group were significantly lower(P<0.05);Compared to SAP group,the levels of Occludin protein (4.1±0.4vs1.2±0.4)and mRNA(4.2±0.3vs1.3±0.3)in Qingyi particle group were significantly higher(P<0.05). Conclusion Qingyi particles can reduce the SAP rat intestinal mucosa damage degree, the effect may be re⁃lated to enhance intestinal mucosa tight junction associated protein Occludin expression.关键词
清胰颗粒/重症急性胰腺炎/紧密连接蛋白/OccludinKey words
Qing Yi particles/severe acute pancreatitis/tight junction protein/occludin分类
生物科学引用本文复制引用
赵光,张淑坤,崔乃强..清胰颗粒对重症急性胰腺炎大鼠肠黏膜上皮紧密连接的保护作用[J].中国中西医结合外科杂志,2014,(6):611-614,4.
