中国中西医结合外科杂志Issue(3)：270-274,5.DOI:10.3969/j.issn.1007-6948.2015.03.018
益肾通淋方对尿路感染大鼠TLRs mRNA表达的影响
The Effect of Yishen Tonglin Decoction on Urinary Tract Infection Rats on Toll-Like Receptors(TLRs) mRNA Expression
摘要
Abstract
Objective To evaluate the effect of Yishen Tonglin Decoction on TLR2 and TLR4 mRNA ex⁃pression of urinary tract infection rats’kidney and bladder tissue. Methods Thirty female SD rats were ran⁃domly divided into 3 groups: normal group,model group and Yishen Tonglin Decoction group. The last two groups were established urinary infection models with bladder injected E.coli concerntration. Blood routine test, urinal leukocyte count and urinal bacterial test were examind on in the first, 7th and 14th days. Body tempera⁃ture was tested every day. Pelvic and bladder tissue were taken 14 days after the tests of the TLR2 and TLR4 mRNA. Results Compared to the normal group,the blood white cell of the model group decreased on the first and 7th days(P<0.05), the urinal white cell count and urinal bacterial count of the model group were all in⁃creased on the first, 7th and 14th days(P<0.05);Compared to the Yishen Tonglin Decoction group, the blood white cell counts of the model group increased on the 1st and 7th days(P<0.05). The urinal white cell and uri⁃nal bacterial counts increased on the first,7th and 14th days(P<0.01);The normal groups’kidney tissue TLR2 mRNA was 1.03 ± 0.41, TLR4 mRNA was 1.66 ± 1.01, blader tissue TLR2 mRNA was 0.23 ± 0.30, TLR4 mRNA was 0.20 ± 0.30;the model groups’kidney tissue TLR2 mRNA was 2.83 ± 3.00, TLR4 mRNA was 2.97 ± 1.85; the blader tissue TLR2 mRNA 0.43±0.06, TLR4 mRNA 2.97±1.85. The Yishen Tonglin Decoction groups’kidney tis⁃sue TLR2 mRNA was 1.20 ± 0.47, TLR4 mRNA was 1.66 ± 0.95; the blader tissue TLR2 mRNA was 0.29 ± 0.03, TLR4 mRNA was 0.22 ± 0.71. Compared to the model group,the TLR2/TLR4 mRNA of the model group was significantly increased(P < 0.05). The TLR2/TLR4 mRNA of the Yishen Tonglin decoction was signifi⁃cantly decreased(P<0.05). Conclusions Yishen Tonglin Decoction can down-regulate the pelvic and blad⁃der TLR2、TLR4 mRNA expression of the urinary infection rats.关键词
益肾通淋方/尿路感染/大鼠/TOLL受体Key words
Yishen Tonglin decoction/urinary tract infection/rats/toll-like receptors分类
生物科学引用本文复制引用
甘澍,王树声,林晓聪,欧阳孔权,李思逸,胡萍,陈志强..益肾通淋方对尿路感染大鼠TLRs mRNA表达的影响[J].中国中西医结合外科杂志,2015,(3):270-274,5.基金项目
广东省中医药局课题（20121173）；广东省中医院朝阳人才专项 （）
