中国执业药师Issue(1)：27-29,3.DOI:10.3969/j.issn.1672-5433.2015.01.006
HPLC测定利胆消石颗粒中槲皮素和山奈素含量
Content Determination of Quercetin and Kaempferol in Lidan Xiaoshi Granules by HPLC
刘译1
作者信息
- 1. 怀化市食品药品检验所，湖南 怀化 418000
- 折叠
摘要
Abstract
Objective: To establish a HPLC method for the content determination of quercetin and kaempferol in lidan xiaoshi granules. Methods: DIKMA-C18 (4.6 mm × 250 mm;5 μm)column was used with the mobile phase of methanol-0.4%phosphoric acid solution (45∶55), the flow rate was 1.0 mL/min, the detection wavelength was at 360 nm and the column temperature was at 30 ℃. Results: A good linear relationship of quercetin and kaempferol was within the range of 0.988~98.8μg/mL (r=0.999 8) and 0.979~97.9μg/mL (r=0.999 7) respectively, and the average recovery of quercetin and kaempferol was 98.21%and 98.18% (RSD was 0.71%and 0.56%). Conclusion: The method is simple and accurate, which can be used for the quality control of lidan xiaoshi granules.关键词
利胆消石颗粒/槲皮素/山奈素/含量测定/高效液相色谱法Key words
Lidan Xiaoshi Granules/Quercetin/Kaempferol/Content Determination/HPLC引用本文复制引用
刘译..HPLC测定利胆消石颗粒中槲皮素和山奈素含量[J].中国执业药师,2015,(1):27-29,3.
