中国执业药师Issue(6)：17-20,4.DOI:10.3969/j.issn.1672-5433.2015.06.004
HPLC考察燕窝加工前后唾液酸的含量变化
Study on the Content Changes of Sialic Acid in Bird ’ s Nest before and after Process by HPLC
卓丹如 1阙慧卿 2林绥 2章宁 1钱丽萍 2林善财1
作者信息
- 1. 福州瑞来春堂生物科技有限公司，福建 福州 350001
- 2. 福建省医学科学研究院
- 折叠
摘要
Abstract
Objective:To establish the separation and analysis methods of sialic acid in bird’s nest and investigate the content changes of sialic acid in raw material(dry bird’s nest after removal of impurities)and processed products of bird’s nest.Methods:Sialic acid(that is N-acetyl neuraminic acid)in bird’s nest was free from the combination of sialic acid glycoprotein through phosphoric acid hydrolysis. The content of sialic acid was determined by HPLC,and the evaluation was made on the fingerprints of three samples using theSimilarity EvaluationSystemforChromatographicFingerprintofTCM,and the content changes of sialic acid were compared. Results:Sialic acid content of bird’s nest raw material was 9.898% and which was 9.921% and 9.913% after process by stewing and making into canned bird’s nest,which was a little higher than the bird’s nest raw material.Conclusion:The content of sialic acid of processed bird’s nest was slightly higher than that of bird’s nest raw materials. It is indicated that sialic acid content can be better preserved in the ready-to-eat canned bird’s nest produced with modern technology.关键词
燕窝原料/炖煮燕窝/燕窝即食罐头/唾液酸(N-乙酰神经氨酸)/高效液相色谱法/中药色谱指纹图谱相似度评价Key words
Bird’s Nest Raw Material/Stewed Bird’s Nest/Ready-To-Eat Canned Bird’s Nest/Saliva Acid (N-Acetyl Neuraminic Acid)/HPLC/Similarity Evaluation of TCM Chromatographic Fingerprint引用本文复制引用
卓丹如,阙慧卿,林绥,章宁,钱丽萍,林善财..HPLC考察燕窝加工前后唾液酸的含量变化[J].中国执业药师,2015,(6):17-20,4.
