中国中医药信息杂志Issue(6)：38-40,3.DOI:10.3969/j.issn.1005-5304.2014.06.014
五子衍宗方对环磷酰胺致小鼠DNA损伤的影响
Effect of Wuzi Yanzong Fang Against Cyclophosphamide Induced DNA Damage in Mice
摘要
Abstract
Objective To study protective effects of Wuzi Yanzong Fang on DNA damage induced by cyclophosphamide (CTX) in mice, and explore its mechanism. Methods BalB/c mice were randomly divided into normal group, model group, Wuzi Yanzong Fang low dose group and Wuzi Yanzong Fang high dose group. Mice in Wuzi Yanzong Fang groups were pretreated with Wuzi Yanzong Fang for 7 days, then the mice in Wuzi Yanzong Fang groups and model group were intraperitoneally injected with CTX (100 mg/kg) every other day for three times, and mice in Wuzi Yanzong Fang groups were continued administered with Wuzi Yanzong Fang. Animals were sacrificed in twelve hours after the final treatment of CTX. ELISA was used to detect 8-OHdG content in serum, and single cell gel electrophoresis to detect DNA damage in bone marrow cells. Results Wuzi Yanzong Fang low dose group and high dose group reduced the level of 8-OHdG in serum. Wuzi Yanzong Fang significantly decreased Olive tail moment, tail moment, tail length and tail DNA%in mouse bone marrow cells. Conclusion Wuzi Yanzong Fang has good protective effects on DNA damage caused by CTX.关键词
五子衍宗方/环磷酰胺/DNA损伤/小鼠Key words
Wuzi Yanzong Fang/cyclophosphamide/DNA damage/mice分类
医药卫生引用本文复制引用
刘苗苗,袁丁,黄威峰,彭奔,张长城..五子衍宗方对环磷酰胺致小鼠DNA损伤的影响[J].中国中医药信息杂志,2014,(6):38-40,3.基金项目
国家自然科学基金(81173375) （81173375）
