中国中医药信息杂志Issue(6)：49-52,4.DOI:10.3969/j.issn.1005-5304.2014.06.017
补阳还五汤对大鼠局灶性脑缺血损伤血脑屏障的影响
Effect of Buyang Huanwu Decoction on Blood-brain Barrier of Focal Crebral Ischemia ;Rats
摘要
Abstract
Objective To investigate the effect of Buyang Huanwu Decoction on blood-brain barrier of focal cerebral ischemia rats, and explore the mechanism of the decoction. Methods The model of focal cerebral ischemia was made by thread embolism method. SD rats were divided randomly into sham-operated group, model group and Buyang Huanwu Decoction group. Buyang Huanwu Decoction group was given Buyang Huanwu Decoction by gavage, the sham-operated group and model group were given normal saline of the same quantity 24 h after modeling. The nervous function deficit scores was evaluated, brain tissues and serum were taken from the rats after treating for seven days, infarct volume was detected by TTC staining, and pathological changes of microvessel were observed microscopically in HE stained sections. And the protein level of MMP-9, MMP-2, VEGF in brain tissue and the serum levels of vWF in serum of every groups were measured by ELISA. Results Compared with model group, Buyang Huanwu Decoction significantly improved the neurological behavior performance, decreased the cerebral infarct volume, alleviated the pathological changes and decreased the protein level of MMP-9, MMP-2, VEGF, vWF. Conclusion Buyang Huanwu Decoction has the protective effect on blood-brain barrier in the model rats of focal cerebral ischemia. The mechanism may be related with restrainning the expression of MMP-9, MMP-2, VEGF, vWF.关键词
补阳还五汤/局灶性脑缺血/血脑屏障/基质金属蛋白酶-2/基质金属蛋白酶-9/血管内皮生长因子/血管性血友病因子/大鼠Key words
Buyang Huanwu Decoction/focal cerebral ischemia/blood-brain barrier/MMP-2/MMP-9/VEGF/von Willebrand factor/rats分类
医药卫生引用本文复制引用
饶晓,汤轶波,潘彦舒,刘希伟,刘洋,池名,马英姿,牟天龙..补阳还五汤对大鼠局灶性脑缺血损伤血脑屏障的影响[J].中国中医药信息杂志,2014,(6):49-52,4.基金项目
国家自然科学基金重点项目(30830120) （30830120）
