中国中医药信息杂志Issue(6)：65-68,75,5.DOI:10.3969/j.issn.1005-5304.2014.06.021
宣肺通腑方对脂多糖致急性肺损伤大鼠肺组织髓样分化蛋白-2、核因子-κB表达的影响
Effects of Xuanfeitongfufang Decoction on Expressions of MD-2 and NF-κB in Lung of Rats ;with Acute Lung Injury Caused by Lipopolysaccharide
摘要
Abstract
Objective To investigate the effects of Xuanfeitongfufang Decoction on expressions of MD-2, NF-κB protein and its mRNA in lung of the rats with acute lung injury caused by LPS. Methods Wistar rats were randomly divided into normal group, model group, dexamethasone group and Xuanfeitongfufang Decoction large-, medium-, small-dose group, each group had eight rats. The ALI rat model was established by LPS tail-intravenous injection (6 mg/kg). The rats in Xuanfeitongfufang Decoction groups were pretreated by Xuanfeitongfufang Decoction (15.12, 7.56, 3.78 g/kg) for 3 days before LPS induced ALI. The rats in dexamethasone group were pretreated by dexamethasone (5 mg/kg). MD-2, NF-κB protein and its mRNA were measured by immunohistochemistry and PCR. The histopathology of the lung injury was observed by light microscope. Results Compared with normal group, the expression of MD-2, NF-κB protein and its mRNA were obviously increased in model group (P<0.01). Compared with model group, the expression of MD-2, NF-κB protein and its mRNA were obviously decreased in Xuanfeitongfufang Decoction groups and dexamethasone group (P<0.01), and there was no obvious difference between Xuanfeitongfufang Decoction groups and dexamethasone group. Light microscope observation indicates that there were large areas of pulmonary hemorrhage and necrosis in model group. While in Xuanfeitongfufang Decoction group and dexamethasone group, the pathological manifestations were much more ameliorated than those of the model group. The lung bronchiale inflammation appeared occasionally, and the edema was lightly. Conclusion Xuanfeitongfufang Decoction can lessen the injury of lung tissue and has protective effects on rats with ALI, the mechanism is possibly related to the inhibition of the expressions of MD-2 and NF-κB protein and its mRNA in injured lung tissues.关键词
宣肺通腑方/急性肺损伤/髓样分化蛋白-2/核因子-κB/蛋白表达/基因表达/大鼠Key words
Xuanfeitongfufang Decoction/acute lung injury/MD-2/NF-κB/protein expression/mRNA expression/rats分类
医药卫生引用本文复制引用
阮琼,张海英,杨爱东,王利霞,吴中华,符胜光,庞慧芳..宣肺通腑方对脂多糖致急性肺损伤大鼠肺组织髓样分化蛋白-2、核因子-κB表达的影响[J].中国中医药信息杂志,2014,(6):65-68,75,5.基金项目
国家中医药管理局重点学科项目(2009年)；上海市重点学科建设资助项目(S30301)；上海市教委创新及预算内科研项目(11YZ67、2011JW89)；上海中医药大学名师研究室、中医临床基础学科建设及基础医学院攀登计划(Z1301011310、12ZLJ08、C0010112) （2009年）
