中国中医药信息杂志Issue(6)：89-94,6.DOI:10.3969/j.issn.1005-5304.2014.06.028
LC-MS/MS同时定量测定大鼠血浆中复方血栓通胶囊的8种入血成分
Simultaneous Determination of Eight Effective Compounds of Fufang Xueshuantong ;Capsule in Rat Plasma by LC-MS/MS
摘要
Abstract
Objective To establish a sensitive and specific LC-MS/MS method for measurement of notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rd, tanshinone Ⅰ, astragaloside Ⅳ and harpagosidein of Fufang Xueshuantong Capsule in rat plasma. Methods The HPLC separation was performed on Thermo Hypersil GOLD column (2.1 mm× 100mm, 5 μm) at 30 ℃, injecting 10 μL and using acetonitrile-water (0.1% formic acid) as the mobile phrase (B was acetonitrile, A was 0.1%formic acid;0-10 min, 25%-55%B;10-20 min, 55%-70%B) with the flow rate of 0.2 mL/min. Detection was performed on a tandem quadrapole mass spectrometer using positive electrospray ionization, SRM scan mode. Results The eight compounds showed good linearity in wide ranges (notoginsenoside R1 1.00-800 ng/mL, ginsenoside Rg1 0.950-760 ng/mL, ginsenoside Re 1.44-1440 ng/mL, ginsenoside Rb1 1.33-1330 ng/mL, ginsenoside Rd 9.90-990 ng/mL, harpagosidein 1.01-1010 ng/mL, astragaloside Ⅳ 1.16-928 ng/mL, tanshinone Ⅰ 10.0-800 ng/mL). In addition, the accuracy and recovery were around 85%-115%and 50%-70%. The RSD of intra and inter day precision were lower than 15%. Conclusion The method is specific, rapid and sensitive. Therefore, it can be applied to pharmacokinetic study of eight effective compounds in Fufang Xueshuantong Capsule.关键词
复方血栓通/三七皂苷R1/人参皂苷Rg1/人参皂苷Rb1/液相色谱-质谱/质谱Key words
Fufang Xueshuantong Capsule/notoginsenoside R1/ginsenoside Rg1/ginsenoside Rb1/LC-MS/MS分类
医药卫生引用本文复制引用
王源,李梦怡,马长华,黄建梅,李莉,马恺悦,冯孟鑫..LC-MS/MS同时定量测定大鼠血浆中复方血栓通胶囊的8种入血成分[J].中国中医药信息杂志,2014,(6):89-94,6.基金项目
国家科技重大专项-重大新药创制(2011ZX09201-201-22) （2011ZX09201-201-22）
