中国中医药信息杂志Issue(7)：32-35,4.DOI:10.3969/j.issn.1005-5304.2014.07.010
竹节参总提物对D-半乳糖致衰老小鼠的影响
Effects of TotalPanax Japonicus Extract onD-galactose Induced Aging Mice
摘要
Abstract
Objective To research the protective effects of totalPanax Japonicus extract on learning memory, antioxidation, and anti-apoptosis of aging mice,and explore the mechanism. Methods Male mice were randomly divided into normal group, model group, Vitamin E (VE) group, Panax Japonicus extract low and high dose group. Except for the normal group, the other groups were injected withD-gal on the back of the neck subcutaneously to establish aging model. Normal group and model group were given a gavage with saline and each treatment group was given a gavage with totalPanax Japonicus extract and VE once a day for 7 weeks after the aging model established. All mice were be measured their learning and memory ability in the eighth week. After the test, the morphological changes of CA1 neurons were observed by HE stain. SOD, GSH-Px, MDA levels in brain tissue were measured by biochemical method, the expressions of Bcl-2 and Bax mRNA were evaluated by RT-PCR.Results Mice inPanax Japonicus extract low and high dose group could spend less time in searching for the platform, improve the learning and memory ability. TotalPanax Japonicus extract increased the activity of SOD and GSH-Px, while decreased the content of MDA. In addition, it could increase the expression level of Bcl-2 mRNA and reduce the expression level of Bax mRNA as well.Conclusion TotalPanax Japonicus extract has anti-aging effect.关键词
竹节参总提物/D-半乳糖/抗衰老/抗氧化/小鼠Key words
totalPanax Japonicus extract/D-gal/anti-aging/antioxidantion/mice分类
医药卫生引用本文复制引用
万静枝,袁丁,狄国杰,张长城,刘朝奇,周志勇,王婷..竹节参总提物对D-半乳糖致衰老小鼠的影响[J].中国中医药信息杂志,2014,(7):32-35,4.基金项目
国家自然科学基金(81100957、81374001、31070314)；湖北省自然科学基金(2010CDB10703) （81100957、81374001、31070314）
