中国中医药信息杂志Issue(8)：42-45,4.DOI:10.3969/j.issn.1005-5304.2014.08.012
鳖甲寡肽I-C-F-6对四氯化碳诱导大鼠肝纤维化的影响
Effects of Oligo-peptide I-C-F-6 in Carapax Trionycis on Rats with Liver Fibrosis Induced by CCl4
摘要
Abstract
Objective To study effects of oligo-peptide I-C-F-6 in carapax trionycis on rats with liver fibrosis induced by CCl4;To discuss its anti-liver fibrosis effects and possible mechanisms. Methods Forty-eight SD male rats were randomly divided into normal control group, model group, bifendate group, and oligo-peptide I-C-F-6 group, 12 in each group.CCl4 was injected intraperitoneally to build rat liver fibrosis model.Oligo-peptide I-C-F-6 group and bifendate group were given subcutaneous injection of oligo-peptide I-C-F-6 (0.12μg/g) or bifendate (0.12μg/g). At the same time, normal control group and model group were giventhe same volume of saline for seven weeks. The levels ofALT, AST,MDA, SOD, IL-4, IL-10 and TNF-α were tested.The histomorphology changes were observed under optical microscopeby HE, and the expressions of transforming growth TGF-β1 were determined by immunohistochemistry.Results Compared with model group, serum levels of ALT and AST were reduced evidently in oligo-peptide I-C-F-6 group. Hepatic content of MDA, IL-4 and TNF-α decreased, while SOD activity and IL-10 were found significantly increased. Liver fibrosis was ameliorated significantly. Hepatic expressions of TGF-β1 were weakly positive.Conclusion Oligo-peptide I-C-F-6 can ameliorate hepatocyte damage of model rats, thus it has anti-oxidative and anti-liver fibrosis effects on liver fibrosis in rats.关键词
鳖甲/寡肽I-C-F-6/抗氧化/肝纤维化/大鼠Key words
carapax trionycis/oligopeptide I-C-F-6/anti-oxidative/liver fibrosis/rats分类
医药卫生引用本文复制引用
王咪娜,林锦璇,杨莹,徐士勋,张华铮,任丽薇,雷海民,张宇忠..鳖甲寡肽I-C-F-6对四氯化碳诱导大鼠肝纤维化的影响[J].中国中医药信息杂志,2014,(8):42-45,4.基金项目
国家自然科学基金(81073017)；北京中医药大学科研创新团队项目(2011-CXTD-15) （81073017）
