中国中医药信息杂志Issue(9)：46-49,4.DOI:10.3969/j.issn.1005-5304.2014.09.014
补阳还五汤及其精简方抗脑缺血损伤功效比较
Comparison of the Effects of Buyang Huanwu Decoction and Its Recipe Composition on Cerebral Ischemic Injury
摘要
Abstract
Objective To investigate the preventive effects of Buyang Huanwu Decoction (BYHWD) and its recipe composition (BYJJF) in focal ischemic brain injury condition in vivo/in vitro. Methods In vivo studies, SD rats were divided into sham-operation group, MCAO group, BYHWD group and BYJJF group based on rat weight, 10 rats in each group. The body weight, infarct area and brain water contents were determined. In vitro studies, H2O2 was used to damage PC12 cells, and the vitro oxidative stress cell model was established. PC12 cells were divided into normal group, blank control group, BYHWD and BYJJF groups with different concentrations (0.1, 0.2, 0.5, 1.0, 2.0, 3.5 mg/mL). MTT method was employed to determine the protective effects of BYHWD and BYJJF on model cells.Results Vivo studies showed that after 7 days of treatment with BYHWD and BYJJF, those determinated quotas were all significantly improved compared with MCAO model rats (P<0.05), and there were no differences between BYHWD and BYJJF (P>0.05).Vtiro studies showed that the protective effects of BYHWD and BYJJF took place 2 hours later, and it was obvious in oxidative stress injury caused by H2O2, with statistical differences with model group (P<0.05). BYHWD and BYJJF could increase cell viability, and there was no difference between the groups with same concentration (P>0.05).Conclusion The research confirmed that BYJJF plays a significant role in improving the cerebral ischemia injury, which is the same performance as BYHWD, and BYJJF can save TCM resources under the precondition of TCM efficacy.关键词
补阳还五汤/补阳还五汤精简方/脑缺血/局灶性脑缺血大鼠模型/氧化应激细胞模型Key words
Buyang Huanwu Decoction/recipe composition of Buyang Huanwu Decoction/cerebral ischemic injury/MCAO rat model/oxidative stress cell model分类
医药卫生引用本文复制引用
刘芳,刘青萍,王宇红,蔡光先,夏相宜,邵乐,柳绍龙..补阳还五汤及其精简方抗脑缺血损伤功效比较[J].中国中医药信息杂志,2014,(9):46-49,4.基金项目
国家重点基础研究发展计划(2012CB723503)；湖南省中药粉体与创新药物省部共建国家重点实验室培育基地开放基金(ZYFT201307)；湖南省教育厅科研项目(13C672、13C683)；湖南省中医药管理局项目(2014135) （2012CB723503）
