中国中医药信息杂志Issue(10)：50-53,4.DOI:10.3969/j.issn.1005-5304.2014.10.015
脱氢紫堇碱对高糖培养心肌成纤维细胞增殖和胶原分泌的影响
Effects of Dehydrocorydaline on Proliferation and Collagen Secretion of Cardiac Fibroblasts Cultured by High Glucose
摘要
Abstract
Objective To observe the effects of dehydrocorydaline (DHC) on proliferation and collagen secretion of cardiac fibroblasts (CFs) cultured by high glucose;To provide experimental evidence for clinical application of Rhizoma Corydalis. Methods CFs cultured in vitro with high glucose were made into models. Collagenase and trypsin were used for the combine digestion of CFs from ratsbornin 24 h. 2-4 generation CFs were cultured by high glucose (25 mmol/L), and then 100, 50, 25 mg/L dethydrocorydaline was added for intervention. Cellular morphology of CFs was observed after 24, 48 h. CFs proliferation was detected by MTT method. Cell cycle was assessed via flow cytometry. The levels of collagen Ⅰ and collagen Ⅲ were determined by ELISA. Results CFs began to grow adherence 3 hours after planting, and CFs cultured by high glucose significantly proliferated 24, 48 h later (P<0.05, P<0.01). The percentage of S+G2+M phase CFs increased significantly after 48 h (P<0.01). The secretion of collagen Ⅰ and collagen Ⅲ also increased significantly (P<0.01). After the intervention of DHC, CFs proliferation was significantly inhibited (P<0.01);the percentage of S+G2+M phase CFs decreased (P<0.01);the secretion of collagen Ⅰ and collagen Ⅲ was reduced (P<0.05, P<0.01). Conclusion DHC can reduce CFs proliferation, decrease collagen secretion of CFs cultured by high glucose, and has potential effects of anti-myocardial fibrosis.关键词
脱氢紫堇碱/体外高糖培养/心肌成纤维细胞/细胞增殖/胶原Key words
dehydrocorydaline/cultured in vitro with high glucose/cardiac fibroblasts/cell proliferation/collagen分类
医药卫生引用本文复制引用
韩路拓,杨佳妹,任钧国,李鸿海,李军梅,刘建勋..脱氢紫堇碱对高糖培养心肌成纤维细胞增殖和胶原分泌的影响[J].中国中医药信息杂志,2014,(10):50-53,4.基金项目
国家自然科学基金(H2809) （H2809）
