中国中医药信息杂志Issue(10)：74-76,3.DOI:10.3969/j.issn.1005-5304.2014.10.022
离子色谱法测定大蒜多糖的单糖组成和含量
Determination of Composition and Content of Monoses in Garlic Polysaccharide by Ion Chromatography
摘要
Abstract
Objective To establish a method to analyze the composition and content of monoses in the garlic polysaccharide by ion chromatography. Methods The monoses in the solution of garlic polysaccharide hydrolysised by trifluoroacetic acid were determined by amino PAC column, eluted by gradient hydroxide sodium and identified by ampere detector (AU as working electrode, Ag/AgCl as reference electrode). Results The garlic polysaccharide was mainly composed by arabinose, galactose, glucose, xylose and fructose. Good resolution could be achieved among the above mentioned five kinds of monoses. Their linear correlations over the investigated concentration were above 0.998, while the average recovery rates were in the range of 95%-105%. Conclusion The ion chromatography method is fast, accurate, simple and reliable, and can be applied in the content determination and quality control of garlic polysaccharide.关键词
离子色谱法/大蒜多糖/单糖/半乳糖/葡萄糖/甘露醇/果糖Key words
ion chromatography/garlic polysaccharide/monose/galactose/glucose/xylose/fructose分类
医药卫生引用本文复制引用
柏冬,韩春霞,王瑞海,刁作彬,刘丽梅..离子色谱法测定大蒜多糖的单糖组成和含量[J].中国中医药信息杂志,2014,(10):74-76,3.基金项目
国家科技重大专项-新药创制平台中药新药探索性课题(2009ZX09301-005-11) （2009ZX09301-005-11）
