中国中医药信息杂志Issue(10)：89-91,3.DOI:10.3969/j.issn.1005-5304.2014.10.027
HPLC测定贯叶金丝桃滴丸中绵马贯众素含量
Content Determination of Dryocrassine in Guanye Jinsitao Drop Pills by HPLC
摘要
Abstract
Objective To develop an assay of HPLC for the content determination of dryocrassine in Guanye Jinsitao drop pills. Methods The column was Kromasil-C18 (150 mm×4.6 mm, 5μm);mobile phase was acetonitrile-chloroform-isopropanol-0.1% phosphoric acid (52∶8∶30∶10);flow rate was 1.0 mL/min;UV detection was performed at 285 nm;detected drift tube temperature was set at 25 ℃. Results The calibration curves were linear in the range of 0.236-2.124 μg for dryocrassine (r=0.999 9). The average recovery rate of dryocrassine was 99.64% (RSD=1.90%). Conclusion The method is simple and accurate. It is suitable for the determination of dryocrassine in Guanye Jinsitao drop pills.关键词
贯叶金丝桃滴丸/绵马贯众素/高效液相色谱法Key words
Guanye Jinsitao drop pills/dryocrassine/HPLC分类
医药卫生引用本文复制引用
周鹏,曹广尚..HPLC测定贯叶金丝桃滴丸中绵马贯众素含量[J].中国中医药信息杂志,2014,(10):89-91,3.基金项目
国家科技重大专项-重大新药创制(2009ZX09102-142)；山东省科技发展计划(2012GGB14018) （2009ZX09102-142）
