中国中医药信息杂志Issue(11)：48-51,4.DOI:10.3969/j.issn.1005-5304.2014.11.015
康莱特注射液对人乳腺癌MCF-7细胞增殖和凋亡的影响
Effect of Kanglaite Injection on Proliferation and Apoptosis of Human Breast Cancer MCF-7 Cells
摘要
Abstract
Objective To investigate effects of Kanglaite injection on proliferation, cycle and apoptosis of human breast cancer MCF-7 cells;To discuss its relevant mechanism. Methods Logarithmic growth phase cells were divided into control group and Kanglaite-treatment group (10, 20, 40μL/mL). Cells were cultured in RPMI-1640 for 24 h before drug treatment. The inhibition rate of Kanglaite injection on proliferation of human breast cancer MCF-7 cells was detected by MTT assay. Apoptosis and cell cycle of MCF-7 cells were detected by flow cytometry. Changes in cell nucleus were determined by Hochest staining assay. Protein expressions of Bcl-2 and Bax were detected by ELISA and Western blot. Results Kanglaite injection for 12 h, 24 h or 48 h resulted in a significant inhibition of MCF-7 cells proliferation (P<0.05, P<0.01);Compared with the control group, Kanglaite injection-treated cells showed increased percentage in G2/M and G0/G1 phases (P<0.001, P<0.01), but showed decreased percentage in S phase (P<0.01), and apoptosis rate increased (P<0.05, P<0.001). Kanglaite injection significantly decreased protein expression of Bcl-2, and enhanced protein expression of Bax of MCF-7 cells (P<0.01, P<0.001). Conclusion Kanglaite injection can inhibit the proliferation of human breast cancer MCF-7 cells, decrease cell cycle and induce apoptosis, the mechanism is related with decreasing protein expression of Bcl-2 and enhance the protein expression of Bax.关键词
康莱特注射液/MCF-7细胞/细胞增殖/细胞周期/细胞凋亡/Bcl-2蛋白/Bax蛋白Key words
Kanglaite injection/MCF-7 cell/cell proliferation/cell cycle/cell apoptosis/Bcl-2分类
医药卫生引用本文复制引用
陈军,马岩,李榆,秦龙,姚莉..康莱特注射液对人乳腺癌MCF-7细胞增殖和凋亡的影响[J].中国中医药信息杂志,2014,(11):48-51,4.基金项目
中央高校基本科研业务费专项资金(lzujbky-2013-216) （lzujbky-2013-216）
