中国中医药信息杂志Issue(12)：51-54,4.DOI:10.3969/j.issn.1005-5304.2014.12.015
叶下珠提取物对急性乙型肝炎小鼠乙型肝炎病毒复制及其抗原表达的影响
Effects of Extract from Phyllanthus urinaria on HBV Replication and Antigen Expression in Mice Model of Acute Hepatitis B Virus Infectio
摘要
Abstract
Objective To observe the effects of the extract from Phyllanthus urinaria (PU) on HBV replication and antigen expression in a murine model of acute hepatitis B virus infection. Methods The eukaryotic expression plasmid HBV1.3, which contained 1.3-fold-overlength genome of HBV, was transfected into C57b/6 mice by hydrodynamic injection via tail vein, to establish a murine model of acute hepatitis B virus infection. The mice were randomly divided into normal group, virus group and PU high, medium and low dosage group. The normal group was injected PBS 1.6 mL per mice. From the 1st day after infection, the mice were treated by intragastric administration with PU at different concentrations for 3 days. On the 4th day after infection, chemiluminescence method was used to detect the concentrations of HBsAg and HBeAg in mice serum;PCR method was used to detect the level of HBV-DNA in serum;immunohistochemistry was used to detect the expressions of HBcAg and HBsAg in mice liver. Results The PU high, medium and low dosage groups all reduced the concentrations of HBsAg and HBeAg in mice sera obviously, compared with the infected mice (P<0.01), in a dosage-dependent manner. The high dosage group dramatically reduced HBV-DNA level, compared with the virus group (P<0.01). The HBV-DNA concentration in medium and low dosage groups decreased, however, there were no significant difference (P>0.05). The drug in high dosage group obviously inhibited the HBcAg and HBsAg expressions in mice liver (P<0.05). Conclusion The PU extract obviously inhibited HBV replication and expression in mice model of acute HBV infection and exerted distinctive anti-HBV effects.关键词
叶下珠提取物/乙型肝炎病毒/复制/表达/水动力法/小鼠Key words
extract from Phyllanthus urinaria/hepatitis B virus/replication/expression/hydrodynamic injection/mice分类
医药卫生引用本文复制引用
吴莹,雷宇,王媛媛,李姝玉,禄颖,王旭丹..叶下珠提取物对急性乙型肝炎小鼠乙型肝炎病毒复制及其抗原表达的影响[J].中国中医药信息杂志,2014,(12):51-54,4.基金项目
教育部新教师基金(20100013120002)；北京中医药大学创新团队项目(2011-CXD-04)；北京中医药大学自主选题项目(JYBZZ-JS017) （20100013120002）
