中国中医药信息杂志Issue(12)：85-87,3.DOI:10.3969/j.issn.1005-5304.2014.12.025
生姜硫磺熏蒸前后HPLC特征色谱图研究
A Study on HPLC Fingerprints of Zingiber Officinale Before and After Sulfur-fumigation
摘要
Abstract
Objective To explain the differences between zingiber officinale and its sulfur fumigation products on chromatography fingerprints by HPLC-DAD;To discuss the influence of sulfur-fumigation on the quality of zingiber officinale. Methods HPLC, diode array detector, and ZORBAX SB-C18 column were used with acetonitrile-water as the mobile phase, gradient elute, volume flow rate of 1 mL/min, column temperature of 25 ℃, and detection wavelength of 280 nm. HPLC-DAD technology was applied to establish the fingerprints of zingiber officinale before and after sulfur-fumigating process, in order to analyze the HPLC fingerprints of zingiber officinale before and after sulfur-fumigating process. External standard method was used to do the quantitative determination of 6-gingerol. Results The 17 common peaks were identified through the comparison of 3 batches of fingerprints of zingiber officinale and their sulfur-fumigated samples. The peak areas of NO.3, NO.10, NO.11, and NO.17 were reduced by 50.68%, 64.41%, 67.68%, and 21.23%respectively. The content of 6-gingerol had no significant change. Conclusion The chemical composition of zingiber officinale changed at different degrees after sulfur-fumigated process. The safety and effectiveness of sulfur fumigation products of zingiber officinale require more researches.关键词
生姜/硫磺熏蒸/6-姜酚/指纹图谱Key words
zingiber officinale/sulfur-fumigate/6-gingerol/fingerprint分类
医药卫生引用本文复制引用
李平,蒋俊,汤坚,汪华君,顾丽亚,李昊天,贾晓斌..生姜硫磺熏蒸前后HPLC特征色谱图研究[J].中国中医药信息杂志,2014,(12):85-87,3.基金项目
镇江市科技支撑计划-社会发展项目(SH2012010)；江苏大学医学临床科技发展基金(JLY20120170) （SH2012010）
